Agarose Gel Electrophoresis/DGGE Protocols

Denaturing Gradient Gel Electrophoresis (DGGE) (Donis-Keller lab)
Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to high denaturant gradient acrylamide gel; initially the fragments move according to molecular weight, but as they progress into higher denaturing conditions, each (depending on its sequence composition) reaches a point where the DNA begins to melt. The partial melting severely retards the progress of the molecule in the gel, and a mobility shift is observed. It is the mobility shift which can differ for slightly different sequences (depending on the sequence, as little as a single bp change can cause a mobility shift). Alleles are detected by differences in mobility.
http://wheat.pw.usda.gov/~lazo/methods/donis/dgge/...
Added: Tue May 14 2002, Hits: 1027, Reviews: 0 Write review Cached
Denaturing Gradient Gel Electrophoresis (DGGE) (electrophoresis.net)
If you are new to DGGE, this will introduce you to this technique.
http://www.electrophoresis.net/tech_dgge.asp
Added: Sun Jun 06 2004, Hits: 747, Reviews: 0 Write review