Overview: Pulse Field Electrophoresis (PFGE) is based on the observation that during continuous field electrophoresis, DNA above 30-50 kb migrates with the same mobility regardless of size. If the DNA is forced to change direction during electrophoresis, different sized fragments begin to separate from each other. PFGE thus allows separation of larger pieces of DNA than conventional agarose gel electrophoresis.
Protocols
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Protocol for Pulsed Field Gel Electrophoresis
(Fuhrman Lab, Department of Biological Sciences, University of Southern California)
http://fuhrmanlab.usc.edu/Documents/Protocols/pfge...
Added: Wed Mar 15 2006, Hits: 3307, Reviews: 0 Write review -
Pulse-Field Gele Electrophoresis
(Dr. Bart Frank, Arthritis and Immunology Program, Oklahoma Medical Research Foundation)
http://omrf.ouhsc.edu/~frank/PFGE.html
Added: Thu Sep 01 2005, Hits: 2722, Reviews: 0 Write review Cached -
Pulsed Field Electrophoresis (PFGE) for Separation of Large DNA
(USDA)
This protocol decribes in great detail the theory, application, instrumentation, separation parameters, sample preparation and receipes.
http://www.nal.usda.gov/pgdic/Probe/v2n3/puls.html
Added: Wed Mar 15 2006, Hits: 2730, Reviews: 0 Write review Cached