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Trouble Shooting DNA electrophoresis

TROUBLESHOOTING DNA AGAROSE GEL ELECTROPHORESIS

Reproduced from Life Technologies, Inc.

Shanta Dube
Technical Services
Life Technologies, Inc.
Rockville, Maryland 20849


If you see faint or no bands on the gel:

If you see smeared DNA bands:

If you see anomalies DNA band migration:

FIGURE 1. The effect of ionic strength on the heat denaturation of l DNA/Hind III fragments. 500 ng DNA/Hind III fragments in NaCl concentrations of 0, 1, 2, 5, 10, 20, 30, and 40 mM were electrophoresed after heating at 65° C for 10 min.