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Trouble Shooting DNA electrophoresis


Reproduced from Life Technologies, Inc.

Shanta Dube
Technical Services
Life Technologies, Inc.
Rockville, Maryland 20849

If you see faint or no bands on the gel:

If you see smeared DNA bands:

If you see anomalies DNA band migration:

FIGURE 1. The effect of ionic strength on the heat denaturation of l DNA/Hind III fragments. 500 ng DNA/Hind III fragments in NaCl concentrations of 0, 1, 2, 5, 10, 20, 30, and 40 mM were electrophoresed after heating at 65° C for 10 min.