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- Agarose gel electrophoresis (basic method) (Matt Lewis, Department of Pathology, University of Liverpool )
Agarose gel electrophoresis is the easiest and commonest way of separating and analyzing DNA. The purpose of the gel might be to look at the DNA, to quantify it or to isolate a particular band. The DNA is visualised in the gel by addition of ethidium bromide. This binds strongly to DNA by intercalating between the bases and is fluorescent meaning that it absorbs invisible UV light and transmits the energy as visible orange light.
Besides a detailed protocol, also mentioned are sample preparation, molecular weight marker and loading buffer. Highly recommended.
http://www.methodbook.net/dna/agarogel.html
Added: Fri Sep 06 2002, Hits: 8119, Reviews: 0 Write review Cached -
Agarose Electrophoresis
(Chen)
DNA electrophoresis, extraction and concentration determination.
http://www.biochem.ucl.ac.uk/~chen/protocols/dnage...
Added: Tue May 14 2002, Hits: 3354, Reviews: 0 Write review -
Agarose Gel
(John Garland, The Biochemistry and Molecular Biology Department of The James H. Quillen College of Medicine in Johnson City, TN)
How to prepare stock solutions and agarose solution
http://qcom.etsu.edu/biochem/protocols/agarose.htm
Added: Tue May 14 2002, Hits: 1116, Reviews: 0 Write review Cached -
Agarose Gel Electrophoresis
(Molecular Biology Cyberlab)
Very basic and illustrated guide
http://www.life.uiuc.edu/molbio/geldigest/electro....
Added: Tue May 14 2002, Hits: 3279, Reviews: 0 Write review Cached -
Agarose Gel Electrophoresis
(Julie B. Wolf, University of Maryland, Baltimore County)
Provides method in gel preparation, recipes of electrophoresis buffer and loading buffer...
http://www.research.umbc.edu/~jwolf/m6.htm#agarose
Added: Tue May 14 2002, Hits: 2692, Reviews: 0 Write review Cached -
Agarose Gel Electrophoresis
(Frank Bottone, Jr.)
http://www.unc.edu/~fbottone/protocol/electro.html
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Agarose Gel Electrophoresis
(Roe, OU)
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 1314, Reviews: 0 Write review Cached -
Agarose Gel Electrophoresis of DNA
(Chen)
Detailed protocol for gel preparation, electrophoresis and DNA extraction from gel.
http://www.biochem.ucl.ac.uk/~chen/protocols/dnage...
Added: Tue May 14 2002, Hits: 2099, Reviews: 0 Write review -
Alkaline Agarose Gel Electrophoresis
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Alkaline agarose gels can be used to determine the size and quality of first and second strand cDNA synthesis by reverse transciptase.
http://www.nottingham.ac.uk/~mbzspd/methods/Alkali...
Added: Sat Apr 10 2004, Hits: 184, Reviews: 0 Write review Cached -
DNA Mobility in Gels
(Roe, OU)
http://www.genome.ou.edu/protocol_book/protocol_ad...
Added: Tue May 14 2002, Hits: 1104, Reviews: 0 Write review Cached -
Electrophoresis of PCR products
(Vodkin Laboratory, University of Illinois)
Describes gel preparation, running and staining
http://soybeangenomics.cropsci.uiuc.edu/protocols/...
Added: Mon Aug 26 2002, Hits: 1381, Reviews: 0 Write review Cached -
Facts and Trouble Shooting of DNA Electrophoresis
(electrophoresis.net)
Fun facts on DNA Electrophoresis and useful trouble shooting
http://www.electrophoresis.net/tech_facts.asp
Added: Sun Jun 06 2004, Hits: 205, Reviews: 0 Write review Cached -
Standard Neutral Agarose Electrophoresis
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Standard agarose gels can be prepared using either TBE or TAE running buffers.
http://www.nottingham.ac.uk/~mbzspd/methods/Standa...
Added: Sat Apr 10 2004, Hits: 289, Reviews: 0 Write review Cached -
Top 10 Fun Facts for DNA Electrophoresis
(Produced from LTI)
Useful tips for preparing agarose gel and electrophoresis
http://www.bio.davidson.edu/courses/Molbio/tips/fu...
Added: Tue May 14 2002, Hits: 1434, Reviews: 0 Write review Cached -
Trouble Shooting DNA Agarose Gels
(Invitrogen)
No band, Smear, anomalies band migration? Find trouble shooting here
http://www.bio.davidson.edu/courses/Molbio/tips/tr...
Added: Tue May 14 2002, Hits: 1727, Reviews: 0 Write review Cached