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  • Lance's Subcloning procedures PDF file (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
    Very informative and useful for all cloning procedures.
    http://www.its.caltech.edu/%7Ebjorker/Martin_subcl...
    Added: Wed Aug 14 2002, Hits: 3199, Reviews: 0  Write review  
  • Subtractive Cloning PDF file (Hazel Sive, MIT)
    Subtractive cloning is a powerful technique that allows isolation of the differences in the nucleic acid composition of two cell samples (Figure 1). Differences can be at the level of RNA species represented within each sample or within the complement of genomic DNAs. Such differences include genes whose differential expression distinguishes one cell type from another, one growth phase from another, or a ormal state from a diseased state. A related procedure, termed positive selection, has been used to isolate differences in cDNA and in genomic DNAs among various genotypes.
    http://web.wi.mit.edu/sive/pub/Lab%20Resources%20D...
    Added: Sat May 24 2003, Hits: 1369, Reviews: 0  Write review  
  • Clone Genes From Phage Libraries (Johnson Lab, Dept. Microbiology & Immunology, UCSF)
    The overall sequence of events is: • Titer and plate out phage • Lift plaques onto filters and prepare them for screening • Make a probe • Hybridize the probe to the filters • Wash the filters and expose to film • Purify putative plaques • Excise plasmid from the desired phage
    http://www.ucsf.edu/micro/faculty/Johnson/protocol...
    Added: Fri Oct 17 2003, Hits: 540, Reviews: 0  Write review   Cached
  • Cosmid cloning (NWFSC)
    Cell preparation, DNA packaging, and Cell Transfection
    http://micro.nwfsc.noaa.gov/protocols/cosmid_packa...
    Added: Tue May 14 2002, Hits: 591, Reviews: 0  Write review   Cached
  • DNA Cloning (Clonit,Worthington)
    Preparation of DNA Fragments for Insertion into Vector
    Preparation of Cleaved Plasmid DNA
    Ligation
    Preparation of Competent Cells
    Transformation
    http://www.worthington-biochem.com/molecBio/clone_...
    Added: Tue May 14 2002, Hits: 4130, Reviews: 0  Write review   Cached
  • DNA Cloning (Fermentas)
    Provides the following details: 1. Cleavage of the DNA vector.
    2. Dephosphorylation of DNA vector cleaved at unique site.
    3. DNA fragment preparation.
    4. Filling-in of 5'-overhangs of the DNA fragment and/or vector termini.
    5. Ligation.
    6. Transformation.
    7. Clone selection.
    http://www.fermentas.com/Profiles/Kits/PDNAClonKit...
    Added: Tue May 14 2002, Hits: 8730, Reviews: 0  Write review   Cached
  • Tips, troubleshooting PJB's Hints for Sub-Cloning PDF file (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
    Very useful tips for cloning
    http://www.its.caltech.edu/%7Ebjorker/PJB%27s_clon...
    Added: Wed Aug 14 2002, Hits: 1595, Reviews: 0  Write review  
  • Quick Shotgun Cloning of Phage Inserts (Schneitz Lab)
    Cloning DNA into phage vector
    http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
    Added: Tue May 14 2002, Hits: 747, Reviews: 0  Write review   Cached
  • Random Subclone Generation (Roe Lab)
    This is a complete protocol for the following procedures
    DNA Sonication
    DNA Nebulization
    Random fragment end-repair, size selection, and phosphorylation
    DNA ligation
    Competent cell preparation
    Bacterial cell transformation
    Microcentrifuge tube transformation
    http://www.genome.ou.edu/protocol_book/protocol_pa...
    Added: Tue May 14 2002, Hits: 2662, Reviews: 0  Write review   Cached
  • Theory, principles and background info Vector Systems for Cloning Different Sizes of DNA (NCBI)
    Cell-based DNA cloning has been used widely as a tool for producing quantities of pure DNA for physical characterization and functional studies of individual genes. However, the size of different DNA sequences of interest can vary enormously. This book chapter describes various vector systems for cloning DNA of different size.
    http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=hmg....
    Added: Sat Jun 12 2004, Hits: 2794, Reviews: 0  Write review   Cached
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