Subcategories
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Protocols
- Construction and Manipulation of Large-Insert Bacterial Clone Libraries (Laboratory for Plant Genomics and Genefinder Genomic Resources, Texas A&M University)
Provide details in the theory and practice for the construction, characterization, and screening of BAC libraries.
http://hbz7.tamu.edu/homelinks/tool/protocol.htm
Added: Tue May 14 2002, Hits: 833, Reviews: 0 Write review Cached - Manual of BAC Protocols (Laboratory for Plant Genomics and Genefinder Genomic Resources, Texas A&M University)
A collection of BAC protocols. All protocols can be downloaded as a word file here
http://hbz7.tamu.edu/homelinks/tool/bac_content.ht...
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BAC End-Sequencing
(Caltech)
Prepare BAC DNA for sequencing
http://www.tree.caltech.edu/protocols/BAC_end_sequ...
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Construction of BAC Library
(Caltech)
Guideline for BAC library preparation, the followings are described in great detail
Preparation of BAC vector DNA
Preparation of source DNA
Ligation and Electroporation
Preparation of BAC DNA
http://wheat.pw.usda.gov/~lazo/methods/caltech/cal...
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DNA Isolation From BAC & PAC Clones
(BACPAC Resource Center (BPRC) at Children's Hospital Oakland Research Institute in Oakland)
This is a rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modification of a standard Qiagen-Tip method that uses no organic extractions or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary.
http://bacpac.chori.org/bacpacmini.htm
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Hybridization of High Density Arrayed BAC Nylon Filter Blots
(Caltech)
Procedures for isolation and purification of cDNA inserts using polymerase chain reaction, probe labeling, probe hybridization and more...
http://www.tree.caltech.edu/protocols/hi_density_s...
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Hybridization of High Density Filters
(BACPAC Resource Center (BPRC) at Children's Hospital Oakland Research Institute in Oakland)
Hybridization of filters can be performed in either rotisserie bottles or heat-seal bags.
http://bacpac.chori.org/highdensity.htm
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Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Followed
(Roe Lab)
http://www.genome.ou.edu/DblAcetateProcV3.html
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Preparation of DNA Template for Direct Sequencing of Large Insert PAC & BAC Plasmids
(BACPAC Resource Center (BPRC) at Children's Hospital Oakland Research Institute in Oakland)
This protocol is designed to allow the use of 96-well trays (8x12 microtiter tray format) and multi-channel devices from the initial inoculation step to the final cycle sequence reaction. It will yield approximately 200ng of purified plasmid per ml of culture fluid. When using one of the accompanying sequencing methods, one cycle sequence reaction will require 160-300ng of template.
http://bacpac.chori.org/dnaprep.htm
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Screening BAC filters with non-radioactive probes
(BACPAC Resource Center (BPRC) at Children's Hospital Oakland Research Institute in Oakland)
Protocol for a single high-density BAC colony filter
http://bacpac.chori.org/nonradioactivehyb.htm
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Standard Operating Procedures for Phage Testing Assay
(BACPAC Resource Center (BPRC) at Children's Hospital Oakland Research Institute in Oakland)
This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard LB agar. If phage is present in a clone streaked or stamped onto the top agarose lawn, the lawn there will be lysed on incubation overnight.
http://bacpac.chori.org/phage_testing_protocol.htm
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