24h Subcloning Protocol
(Mirmira Laboratory at the University of Virginia)
This protocol is designed for subcloning inserts (I) from one vector into another vector (V). The inserts can be anywhere from 30 bp to 8 kb (possibly higher)
Added: Sat Jun 05 2004, Hits: 3514, Reviews: 0
(Donis Keller Lab)
The method is used to clone smaller portions of inserts (up to 15 kb in size) which previously have been cloned in YACs phage cosmids or other plasmids.
Added: Tue May 14 2002, Hits: 2020, Reviews: 0
The following protocols minimize the number of manipulations required to prepare DNA fragments for ligations, thereby both saving time and increasing reliability.
Added: Tue May 14 2002, Hits: 3318, Reviews: 1 Read reviews