Protocols
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Construction of Genome DNA Library
(Michael Blaber's Lab)
Very useful guide and protocol for genome DNA library construction
http://wine1.sb.fsu.edu/bch5425/lect28/lect28.htm
Added: Tue May 14 2002, Hits: 794, Reviews: 0 Write review Cached -
Construction of Lambda DNA Library
(Promega)
This protocol contains detailed procedures for construction of lambda library, including Preparation of Bacterial Culture for Infection, Ligation of Insert to Vector Arms, Packaging and Titration of Ligated DNA, Transfer of Phage to Nitrocellulose Filters for Screening with Nucleic Acid Probes, Filter Preparation for Screening with Nucleic Acid Probes, Lysate Preparation...
http://www.promega.com/tbs/TB005/tb005.html
Added: Tue May 14 2002, Hits: 1619, Reviews: 0 Write review Cached -
Genomic Cloning Technical Manual
(Promega)
Very useful guide for genomic library construction
http://www.promega.com/tbs/tm029/tm029.html
Added: Tue May 14 2002, Hits: 2262, Reviews: 0 Write review Cached -
Lambda vector preparation
(Practical Approach Online, Oxford University Press)
Preparation and purification of lambda EMBL3 vector for construction of genomic DNA libraries
http://www.oup.co.uk/pdf/pas/9v1-7-7.pdf
Added: Sat Aug 17 2002, Hits: 1031, Reviews: 0 Write review -
Preparation and purification of lambda gtWES vector for construction of genomic DNA libraries
(Practical Approach Online, Oxford University Press)
Vector digestion and purification
http://www.oup.co.uk/pdf/pas/9v1-7-6.pdf
Added: Sat Aug 17 2002, Hits: 913, Reviews: 0 Write review