Protocols
- Colony Screening by PCR (Matt Lewis, Department of Pathology, University of Liverpool)
This is the fastest way to screen bacterial colonies
http://www.methodbook.net/pcr/pcrscreen.html
Added: Fri Sep 06 2002, Hits: 3273, Reviews: 0 Write review Cached - Screening Colonies by Hybridization (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Lifting colonies from agar plates onto nitrocellulose membranes, lyse cells, neutralize and bake them with the final result of having the bacterial DNA fixed to the membranes, ready for hybridization.
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Sun Nov 03 2002, Hits: 208, Reviews: 0 Write review Cached -
Colony Cracking: Quick Test for Inserts in Plasmids
(Hideaki Shiraishi, Kyoto University)
. coli cells can be disrupted in an alkaline solution containing detergent. The lysate contains enough DNA to be detected in a single lane of an agarose gel provided that the plasmid have pUC-derived replication origin. This protocol is modified from that described in the first version of "Molecular Cloning"
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
Added: Fri Jan 30 2004, Hits: 1623, Reviews: 0 Write review Cached -
PCR Amplification of Inserts from Bacterial Cultures
(CIMMYT Applied Molecular Genetics Laboratory)
PCR amplification of plasmid insert without plasmid preparation
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Mon Aug 05 2002, Hits: 3944, Reviews: 0 Write review -
PCR from Bacterial Colonies
(Hideaki Shiraishi, Kyoto University)
This procedure is used to analyze inserts in pUC-derived plasimids.
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
Added: Fri Jan 30 2004, Hits: 1286, Reviews: 0 Write review Cached -
Screening clone by PCR
(Herskowitz Lab)
http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
Added: Tue May 14 2002, Hits: 1441, Reviews: 0 Write review