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Protocols
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PCR Typing Ear Punches
(Case Transgenic and Targeting Facility)
http://transgenic.cwru.edu/protocols/pcrtypingearp...
Added: Sun Mar 22 2009, Hits: 140, Reviews: 0 Write review Cached -
AP Assay and FGF Binding
(Dr. Ornitz's Lab, Washington University Medical School)
FGFR-AP-FGF Binding Assay, PCR Assays for Knockout Mice, RNase Protection Assay:The bound receptor was quantified by measuring the initial rate of substrate hydrolysis at 405 nm and same amount of proteins was used in the binding assays...
http://molecool.wustl.edu/ornitzlab/proto.assays.h...
Added: Tue May 14 2002, Hits: 213, Reviews: 0 Write review Cached -
Combined LacZ staining and in situ hybridization
(Practical Approach Online, Oxford University Press)
Mouse embryos staining, including: in vitro transcription of riboprobe, embryo fixation and permeabilization, hybridization and post-hybridization washes, immunodetection and development of signal.
http://www.oup.co.uk/pdf/pas/13-3-9.pdf
Added: Wed Sep 04 2002, Hits: 258, Reviews: 0 Write review -
Detecting Transgenes by PCR and Southern Blot
(Case Transgenic and Targeting Facility)
http://transgenic.cwru.edu/protocols/dnacalc.html
Added: Sun Mar 22 2009, Hits: 325, Reviews: 0 Write review Cached -
DNA Purification for PCR only from yolk sacs, toes, or 2 mm Tail
(U. of Virginia Transgenic Mouse Core Facility)
http://www.med.virginia.edu/medicine/inter-dis/tra...
Added: Tue May 14 2002, Hits: 199, Reviews: 0 Write review Cached -
Embedding, sectioning and counterstaining of LacZ embryos
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-3-13.pdf
Added: Wed Sep 04 2002, Hits: 178, Reviews: 0 Write review -
G-banding for analysing mouse chromosomal rearrangements
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-6a-2.pdf
Added: Wed Sep 04 2002, Hits: 410, Reviews: 0 Write review -
Genomic Southerns of DNA from Tail Clips
(Transgenic and ES Cell Services at OSU)
http://www.med.ohio-state.edu/tafhome/webpages/pro...
Added: Tue May 14 2002, Hits: 106, Reviews: 0 Write review Cached -
Genotyping Transgenic Mice by PCR
(University of Michigan Transgenic Animal Model Core)
Test mice for the presence of the transgene by PCR. It is provided for those investigators who are unfamiliar with mouse genotyping and who need to establish a genotyping method.
http://www.med.umich.edu/tamc/pcr.html
Added: Tue May 14 2002, Hits: 684, Reviews: 0 Write review Cached -
LacZ Cell Staining and β-galactosidase Embryo Staining
(Dr. Ornitz's Lab, Washington University Medical School)
http://molecool.wustl.edu/ornitzlab/proto.LacZ.htm
Added: Tue May 14 2002, Hits: 315, Reviews: 0 Write review Cached -
Mitotic chromosomes from mouse peripheral blood
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-6a-1.pdf
Added: Wed Sep 04 2002, Hits: 396, Reviews: 0 Write review -
Mouse Tail (or organ) Biopsy DNA Extraction
(The Cancer Center at Washington University)
http://medicine.wustl.edu/~escore/htmldocs/tailpre...
Added: Tue May 14 2002, Hits: 152, Reviews: 0 Write review Cached -
Mouse Tail Digestion Procedure
(Pharmacological Sciences Consortium Core)
http://transgenic.cwru.edu/tail.htm
Added: Tue May 14 2002, Hits: 84, Reviews: 0 Write review Cached -
PCR Analysis of Tail DNA
(University of Michigan, Transgenic Animal Model Core)
http://www.med.umich.edu/tamc/pcr.html
Added: Tue May 14 2002, Hits: 238, Reviews: 0 Write review Cached -
PCR of DNA from Ear Clip Tissue
(Transgenic and ES Cell Services at OSU)
http://www.med.ohio-state.edu/tafhome/webpages/pro...
Added: Tue May 14 2002, Hits: 65, Reviews: 0 Write review Cached -
Preparation of Copy Standards for PCR Genotyping
(University of Michigan Transgenic Animal Model Core)
The purpose of spiking tail DNA with a known amount of transgene DNA is to produce copy standards. These are used to verify the sensitivty of your PCR genotyping assay and to establish transgene copy number in Southern blot analysis.
http://www.med.umich.edu/tamc/spike.html
Added: Tue May 14 2002, Hits: 347, Reviews: 0 Write review Cached -
Preparation of Mouse Tail DNA for Dot Blots or PCR
(Eric Mercer)
These procedures were originally devised in Richard Palmiter's lab for use with tail dots (DNA spotted onto a nitrocellulose filter and probed for a transgene; quicker than doing southerns), and subsequently modified for PCR preps.
http://www.muridae.com/wmc/docs/tailDNA_prep.html
Added: Tue May 14 2002, Hits: 209, Reviews: 0 Write review Cached -
RNA Whole Mount In Situ Hybridization
(Cepko/Tabin Lab)
protocol for both mouse and chick embryos
http://axon.med.harvard.edu/~cepko/protocol/ctlab/...
Added: Tue May 14 2002, Hits: 185, Reviews: 0 Write review Cached -
Tail Preps: DNA Isolation From Mouse Tails Without Phenol
(Hedrick Lab, UCSD Cancer Center)
DNA isolation for Southern analysis
http://hedricklab.ucsd.edu/Protocol/TailPreps.html
Added: Tue Dec 10 2002, Hits: 2548, Reviews: 0 Write review Cached -
Whole Mount Human Placental Alkaline Phosphatase Staining
(Nagy Lab)
http://www.mshri.on.ca/nagy/Protocols/APwmstain.ht...
Added: Tue May 14 2002, Hits: 133, Reviews: 0 Write review Cached -
Whole Mount Immunoflurescentstaining
(Nagy Lab)
http://www.mshri.on.ca/nagy/Protocols/wmimmun.htm
Added: Tue May 14 2002, Hits: 107, Reviews: 0 Write review Cached -
Whole Mount LacZ Staining
(Nagy Lab)
http://www.mshri.on.ca/nagy/Protocols/lacZ.htm
Added: Tue May 14 2002, Hits: 251, Reviews: 0 Write review Cached -
whole mount preparation and lacZ staining in the mammary gland
(Laboratory of Genetics & Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH)
The bacterial beta-galactosidase gene lacZ is frequently used as a reporter gene. The expression of transgenic constructs can be monitored by histochemistry with the chromogenic substrate X-gal. This allows precise cellular localization of gene activity.
http://mammary.nih.gov/tools/histological/Histolog...
Added: Thu Jul 25 2002, Hits: 252, Reviews: 0 Write review Cached -
Whole Mount RNA in situ Hybridization
(Nagy Lab)
RNA probe labeling and purification, embryo preparation and more...
http://www.mshri.on.ca/nagy/Protocols/wmrna.htm
Added: Tue May 14 2002, Hits: 193, Reviews: 0 Write review Cached -
X-gal & AP Staining Protocol
(Cepko/Tabin Lab)
Very detailed protocol with background information and several applications.
http://axon.med.harvard.edu/~cepko/protocol/xgalpl...
Added: Tue May 14 2002, Hits: 219, Reviews: 0 Write review Cached