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Human Alkaline Phosphatase Staining

Human Alkaline Phosphatase staining of transgenic embryo/tissue

AP Buffer:


Since this is generally done in conjunction with lacZ staining, embryo/tissue is usually fixed as per lacZ staining. For wholemount staining, penetration of stain may be improved by fixing in PBS + 0.2% glutaraldehyde + 0.02% NP-40 + 0.01% NaDC (at 4oC).