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  • Beta-gal Staining- Whole Mount (Karl Clark)
    Beta-gal staining allows identification of embryonic tissues/cells expressing lacZ marker protein by development of pigmented (blue) product in the presence of lacZ enzymatic activity.
    http://www.cbs.umn.edu/~kclark/protocols/bgal.html
    Added: Tue May 14 2002, Hits: 2131, Reviews: 0  Write review   Cached
  • Immunofluorescence- Whole Mount (Karl Clark)
    Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically to an antigen (e.g. Luciferase); then a secondary antibody conjugated to a flourochrome or enzyme is bound to the heavy chain constant region of the primary antibody with specificity to the species of origin of the primary (e.g. anti-rabbit conjugated to Rhodamine
    http://www.cbs.umn.edu/~kclark/protocols/immuno.ht...
    Added: Tue May 14 2002, Hits: 3658, Reviews: 0  Write review   Cached
  • Immunohistochemistry on Whole-Mount Embryos (Vesicle Trafficking)

    http://cmgm.stanford.edu/~vesicle/protocols/embryo...
    Added: Tue May 14 2002, Hits: 3175, Reviews: 0  Write review   Cached
  • Staining Protocols for Explant Cultures (Aplin Laboratory, Reproductive Biology at the University of Manchester )
    The protocol uses a single primary antibody with nuclear counterstaining, but it can be extended to double antibody staining. It requires an inverted microscope with fluorescence illumination and long working distance objectives.
    http://www.medicine.man.ac.uk/aplin/protocols2.htm...
    Added: Fri Dec 13 2002, Hits: 2298, Reviews: 0  Write review   Cached
  • Whole Mount Staining of Embryos (XMMR)

    http://www.xenbase.org/xmmr/Marker_pages/methods_p...
    Added: Tue May 14 2002, Hits: 2010, Reviews: 0  Write review   Cached
  • Whole-Mount Immunocytochemistry (Klymkowsky Lab)

    http://spot.colorado.edu/~klym/methods.htm#wholemo...
    Added: Tue May 14 2002, Hits: 3822, Reviews: 0  Write review   Cached
  • Wholemount Staining PDF file (Rosen Lab, Department of Molecular and Cellular Biology, Baylor College of Medicine)
    Hematoxylin staining of whole mammary glands
    http://www.bcm.edu/rosenlab/protocols/wholemount.p...
    Added: Sat Aug 24 2002, Hits: 3245, Reviews: 0  Write review