Protocols
-
Beta-gal Staining- Whole Mount
(Karl Clark)
Beta-gal staining allows identification of embryonic tissues/cells expressing lacZ marker protein by development of pigmented (blue) product in the presence of lacZ enzymatic activity.
http://www.cbs.umn.edu/~kclark/protocols/bgal.html
Added: Tue May 14 2002, Hits: 275, Reviews: 0 Write review Cached -
Immunofluorescence- Whole Mount
(Karl Clark)
Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically to an antigen (e.g. Luciferase); then a secondary antibody conjugated to a flourochrome or enzyme is bound to the heavy chain constant region of the primary antibody with specificity to the species of origin of the primary (e.g. anti-rabbit conjugated to Rhodamine
http://www.cbs.umn.edu/~kclark/protocols/immuno.ht...
Added: Tue May 14 2002, Hits: 407, Reviews: 0 Write review Cached -
Immunohistochemistry on Whole-Mount Embryos
(Vesicle Trafficking)
http://cmgm.stanford.edu/~vesicle/protocols/embryo...
Added: Tue May 14 2002, Hits: 2463, Reviews: 0 Write review Cached -
Staining Protocols for Explant Cultures
(Aplin Laboratory, Reproductive Biology at the University of Manchester )
The protocol uses a single primary antibody with nuclear counterstaining, but it can be extended to double antibody staining. It requires an inverted microscope with fluorescence illumination and long working distance objectives.
http://www.medicine.man.ac.uk/aplin/protocols2.htm...
Added: Fri Dec 13 2002, Hits: 2141, Reviews: 0 Write review Cached -
Whole-Mount Immunocytochemistry
(Klymkowsky Lab)
http://spot.colorado.edu/~klym/methods.htm#wholemo...
Added: Tue May 14 2002, Hits: 3096, Reviews: 0 Write review Cached -
Wholemount Staining
(Rosen Lab, Department of Molecular and Cellular Biology, Baylor College of Medicine)
Hematoxylin staining of whole mammary glands
http://public.bcm.tmc.edu/rosenlab/protocols/whole...
Added: Sat Aug 24 2002, Hits: 3061, Reviews: 0 Write review -
Wholemount Staining of Embryos
(XMMR)
http://www.xenbase.org/xenbase/original/WWW/Marker...
Added: Tue May 14 2002, Hits: 1189, Reviews: 0 Write review Cached