Protocols
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3% Trevigel Protocol for 3 bp resolution of SSR PCR products
(Kim Marshall)
http://dendrome.ucdavis.edu/Protocols/3p.html
Added: Tue May 14 2002, Hits: 130, Reviews: 0 Write review Cached -
A Rapid CTAB DNA Isolation Technique Useful for RAPD Fingerprinting and Other PCR Applications
(Kim Marshall)
http://dendrome.ucdavis.edu/Protocols/rapid_ctabdn...
Added: Tue May 14 2002, Hits: 326, Reviews: 0 Write review Cached -
AFLP
(Hubbard Center for Genome Studies)
AFLP is a method for genotyping individuals for a large number of loci using a minimal number of PCR reactions.
http://hcgs.unh.edu/protocol/aflp/
Added: Wed Feb 11 2009, Hits: 656, Reviews: 0 Write review Cached -
Amplified Fragment Length Polymorphisms (AFLPs)
(Oregon State U)
http://www.css.orst.edu/Barley/BREEDING/aflps.htm
Added: Tue May 14 2002, Hits: 194, Reviews: 0 Write review Cached -
Chemiluminescent AFLP protocol
(CIMMYT Applied Molecular Genetics Laboratory)
After amplification and electrophoresis on sequencing gels, the amplification products are transfered to a nylon membrane, and the anti-Dig / alkaline phosphatase and CSPD system is used to detect on an X-ray film the amplification products.
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Fri Aug 02 2002, Hits: 567, Reviews: 0 Write review -
Denaturing Gradient Gel Electrophoresis (DGGE)
(Donis-Keller lab)
Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to high denaturant gradient acrylamide gel; initially the fragments move according to molecular weight, but as they progress into higher denaturing conditions, each (depending on its sequence composition) reaches a point where the DNA begins to melt. The partial melting severely retards the progress of the molecule in the gel, and a mobility shift is observed. It is the mobility shift which can differ for slightly different sequences (depending on the sequence, as little as a single bp change can cause a mobility shift). Alleles are detected by differences in mobility.
http://hg.wustl.edu/hdk_lab_manual/dgge/dgge1.html
Added: Tue May 14 2002, Hits: 572, Reviews: 0 Write review Cached -
RAPD Reaction and Cycling Conditions
(Kim Marshall)
http://dendrome.ucdavis.edu/Protocols/pcrrapd_seed...
Added: Tue May 14 2002, Hits: 177, Reviews: 0 Write review Cached - Single Strand Conformation Polymorphism (SSCP) Analysis by Nondenaturing PAGE
This protocol, with brief principle and procedure can be informative and useful for molecular biology researchers working on genetic polymorphism analysis of QTLs such as Leptin, STAT5A, DGAT and Butyrophilin candidate genes using SSCP technique.
Added: Mon Feb 02 2009, Reviews: 0 Write review -
SSR Gel and Silver Staining Protocol
(Kim Marshall)
http://dendrome.ucdavis.edu/Protocols/silverstain....
Added: Tue May 14 2002, Hits: 214, Reviews: 0 Write review Cached