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Protocols
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Mating Transgenic Mice
(University of Michigan Transgenic Animal Model Core)
http://www.med.ohio-state.edu/tafhome/webpages/pro...
Added: Tue May 14 2002, Hits: 290, Reviews: 0 Write review Cached -
Timeline for Transgenic Mouse Production/Analysis
(University of Michigan Transgenic Animal Model Core)
http://www.med.umich.edu/tamc/tgtime.html
Added: Tue May 14 2002, Hits: 411, Reviews: 0 Write review Cached -
Transgenic Animals
(Brinkmann)
Two techniques are introduced: Microinjection of linear DNA into the pronucleus and Transfer of Embryonic Stem Cells into Blastocysts
http://www.brinkmann.com/ECET_appl5.asp
Added: Tue May 14 2002, Hits: 236, Reviews: 0 Write review Cached -
Transgenic Outline
(University of Michigan Transgenic Animal Model Core)
This is an outline of the steps necessary to obtain a transgenic mice. Simply put, the investigator constructs a transgene with a promoter and a structural gene (for example a reporter gene such as lacZ or a transcription factor). DNA is prepared and microinjected into fertilized mouse eggs. Potentially transgenic mice are born. Transgenic founders are identified and bred to produce offspring for analysis
http://www.med.umich.edu/tamc/tgoutline.html
Added: Tue May 14 2002, Hits: 694, Reviews: 0 Write review Cached -
Transgenic Strain Staining Assay
(Jackson Labs)
Lac-Z Detection in Tail Biopsies
http://www.jax.org/imr/tgr26_staining.html
Added: Tue May 14 2002, Hits: 230, Reviews: 0 Write review Cached