pRARE2 vector sequence - (Nov/23/2008 )

Hi all,
I am looking for the sequence of the pRARE2 vector (the one which is carried inside Rosetta2 cells). I couldn't find it at the more common databases....

-lost higo-

QUOTE (lost higo @ Nov 24 2008, 05:27 AM)

Hi all,
I am looking for the sequence of the pRARE2 vector (the one which is carried inside Rosetta2 cells). I couldn't find it at the more common databases....

What part of the sequence do you need?

-swanny-

I'd like to cut it, so probably the whole map. Unless there is a known enzyme, which cuts only once...

-lost higo-

QUOTE (lost higo @ Nov 26 2008, 04:48 AM)

I'd like to cut it, so probably the whole map. Unless there is a known enzyme, which cuts only once...

Try this paper [attachment=5606:Rogulin_..._cloning.pdf] for some ideas

-swanny-

QUOTE (swanny @ Nov 26 2008, 07:43 AM)

QUOTE (lost higo @ Nov 26 2008, 04:48 AM)

I'd like to cut it, so probably the whole map. Unless there is a known enzyme, which cuts only once...

Try this paper [attachment=5606:Rogulin_..._cloning.pdf] for some ideas

Thanx!

-lost higo-