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Top : New Forum Archives (2009-): : ChIP-and-Next-Generation-Sequencing
61. Ab for acetyl modifications in low-input ChIP experiments - (reply: 1)
62. abcam 209 GFP antibody for CHIP- is there an alternative GFP antibody useful for - (reply: 5)
63. no DNA fragment pull down in the first CpG island (MBD-seq) - (reply: 2)
64. Quantify DNA before ChIP PCR - (reply: 3)
65. ChIP input - sonication problem? - (reply: 6)
66. Sequential ChIP with a repeat of the sonication step? - (reply: 1)
67. ChIP kit recommendations - (reply: 3)
68. What remains after elution? - (reply: 1)
69. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
70. Sonication woes for Chip-seq, using Bioruptor - (reply: 7)
71. Sonication problem with BOTH BioRuptor and Covaris - (reply: 9)
72. Can I go for ChP having frozen tissue - (reply: 4)
73. help-Chip antibody p65 ,NFKB mouse - (reply: 4)
74. Shearing issue: always having unsheared DNA (Please help!) - (reply: 9)
75. Input reverse cross linking - (reply: 1)
76. questions about shearing chromatin analysis - (reply: 3)
77. Advice on sonication (c2c12) - (reply: 12)
78. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
79. Neeeeed Help with my CHIP! Input value so high~ - (reply: 5)
80. Matrix ChIP - No signal - (reply: 9)
81. I forgot treat my samples with proteinase K before the DNA extraction step - (reply: 1)
82. Some ChIP Questions - (reply: 5)
83. Quenching the crosslinking - (reply: 2)
84. RNA immunoprecipitation DNase treatment - (reply: 4)
85. Formaldehyde cross linking problem!!! - (reply: 3)
86. I'd like your advice on NIH 3T3 sonication - (reply: 3)
87. ChIP data normalization - (reply: 2)
88. help on % input calculation please!! - (reply: 1)
89. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
90. ChIP on episomal DNA - (reply: 2)

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