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ChIP-Assay
91.
ChIP-chip - how much DNA do you label? equal amounts?
(reply: 1)
92.
Fast Decrosslinking -
(reply: 3)
93.
Samples for ChIP-seq library preparation - DNA concentrations measurement issues
(reply: 15)
94.
ChIP-seq multiplexing - Anyone done it with human samples?
(reply: 4)
95.
negative control region issues -
(reply: 9)
96.
How can I decrease IgG background? - My IgG background is too high!!!
(reply: 12)
97.
Chelex and Chip-chip -
(reply: 1)
98.
Amount of chromatin for IgG control - URGENT!
(reply: 1)
99.
Amount of fixation buffer - is it dependent on the number of cells?
(reply: 2)
100.
ChIP-Chip vs. ChIP-Seq -
(reply: 1)
101.
good amplification in classic PCR, no amplification in qPCR -
(reply: 6)
102.
DNA sonication - RNA contamination? - New probe, start all over
(reply: 13)
103.
Reference for qPCR Chip? -
(reply: 1)
104.
Is my antibody ChIP grade? -
(reply: 10)
105.
High background with biorupter -
(reply: 7)
106.
Reverse cross-linking in Active Motif kit - only 15 mins? -
(reply: 7)
107.
ChIP data interpretation and representation -
(reply: 1)
108.
Need unusually stringent wash to reduce BG -
(reply: 1)
109.
First ChIP - good start? -
(reply: 4)
110.
ChIP in WT and KO cells - how to analyze -
(reply: 1)
111.
Determing if ChIP Signal Is Real -
(reply: 2)
112.
Dual cross-linking thoughts - Agents to help analyse proteins indirectly bound to DNA
(reply: 15)
113.
Protein G vs Protein A for Rabbit Antibodies -
(reply: 8)
114.
very low ChIP'd DNA amount. -
(reply: 1)
115.
not being able to reproduce result -
(reply: 1)
116.
Magnetic Beads - Incubation -
(reply: 3)
117.
Mock IgG versus No Antibody - Negative Control -
(reply: 6)
118.
weak ChIP signal. Help!!! -
(reply: 2)
119.
White Precipitate Following Sonication -
(reply: 8)
120.
Sonication trouble - Using both Mnase and Sonication to get 200-1000 bp
(reply: 2)
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