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61. ChIP doesn't work. Please help! - Too much "normal rabbit IgG" bockround (reply: 8)
62. Weird Problem with Fast ChIP - (reply: 9)
63. Two questions: Magnetic Bead Aggregation and Chelex in ChIP assay - (reply: 8)
64. help regarding ChIP assay after sonication with bioruptor - (reply: 9)
65. Isoamyl Alcohol in DNA extraction - (reply: 3)
66. Chip-seq blocking reagent - Samlon DNA is useful for Chip-chip (reply: 1)
67. Normalization problem - different transcription factor amounts (reply: 1)
68. ChIP Assay Problems - Receiving poor data from assay (reply: 3)
69. Has anyone ever isolated nuclei prior to fixation and performed a ChIP? - (reply: 5)
70. ChIP fragment issues - (reply: 5)
71. H3Ac and H3 Enrichment - (reply: 7)
72. Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
73. What's a decent %IP yield? - (reply: 2)
74. ChIP library prep for TF - ideas? - How best to scale up ChIP for ChIP-Seq (reply: 4)
75. Typical ChIP yields - (reply: 3)
76. How to prepare LiCl washing liquid - (reply: 2)
77. Fast ChIP protocol - (reply: 3)
78. Is crosslinking necessary in ChIP? - (reply: 1)
79. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
80. Enzymatic shear vs sonication - (reply: 1)
81. How to design primers to check my candidates in a ChiP assay - (reply: 4)
82. no PCR product from input... - (reply: 8)
83. Lysis of the cells - (reply: 1)
84. Cells do not lyse in SDS buffer - (reply: 1)
85. the ChIP agarose gel results - Should the input always be more than the IP one? (reply: 2)
86. How much template DNA do you use for qPCR - (reply: 1)
87. How long samples can be stored - (reply: 3)
88. Generation of ChIP grade antibody - Purification of recombinant protein (reply: 3)
89. Fragment sizes after ChIP - 2 smears: one at 150bp and one at 1000/10000 pb (reply: 2)
90. Eluting from magnetic beads in ChIP. - (reply: 10)
62. Weird Problem with Fast ChIP - (reply: 9)
63. Two questions: Magnetic Bead Aggregation and Chelex in ChIP assay - (reply: 8)
64. help regarding ChIP assay after sonication with bioruptor - (reply: 9)
65. Isoamyl Alcohol in DNA extraction - (reply: 3)
66. Chip-seq blocking reagent - Samlon DNA is useful for Chip-chip (reply: 1)
67. Normalization problem - different transcription factor amounts (reply: 1)
68. ChIP Assay Problems - Receiving poor data from assay (reply: 3)
69. Has anyone ever isolated nuclei prior to fixation and performed a ChIP? - (reply: 5)
70. ChIP fragment issues - (reply: 5)
71. H3Ac and H3 Enrichment - (reply: 7)
72. Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
73. What's a decent %IP yield? - (reply: 2)
74. ChIP library prep for TF - ideas? - How best to scale up ChIP for ChIP-Seq (reply: 4)
75. Typical ChIP yields - (reply: 3)
76. How to prepare LiCl washing liquid - (reply: 2)
77. Fast ChIP protocol - (reply: 3)
78. Is crosslinking necessary in ChIP? - (reply: 1)
79. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
80. Enzymatic shear vs sonication - (reply: 1)
81. How to design primers to check my candidates in a ChiP assay - (reply: 4)
82. no PCR product from input... - (reply: 8)
83. Lysis of the cells - (reply: 1)
84. Cells do not lyse in SDS buffer - (reply: 1)
85. the ChIP agarose gel results - Should the input always be more than the IP one? (reply: 2)
86. How much template DNA do you use for qPCR - (reply: 1)
87. How long samples can be stored - (reply: 3)
88. Generation of ChIP grade antibody - Purification of recombinant protein (reply: 3)
89. Fragment sizes after ChIP - 2 smears: one at 150bp and one at 1000/10000 pb (reply: 2)
90. Eluting from magnetic beads in ChIP. - (reply: 10)