Subcategories
Related Categories:
Protocols
- Western Blotting Protocol (EnCor Biotechnology)
A basic nitrocellulose/PVDF protocol.
http://www.encorbio.com/protocols/blotting.htm
Added: Mon Feb 23 2009, Hits: 30910, Reviews: 0 Write review Cached - Western Blotting with Biotinylated Antibodies (BD Biosciences)
procedures including sample preparation from cell and tissue, quantification, electrophoresis, transfer, semi-dry transfer, blocking and troubleshootings.
http://www.bdbiosciences.com/support/resources/pro...
Added: Wed Aug 28 2002, Hits: 5245, Reviews: 0 Write review Cached - Western Blotting with Monoclonal Antibodies (BD Biosciences)
This is a detailed western protocol using monoclonal antibody for general use.
http://www.bdbiosciences.com/support/resources/pro...
Added: Tue May 14 2002, Hits: 8617, Reviews: 0 Write review Cached - Western Immunoblotting of Proteins (Millipore)
Western immunoblotting protocol for general use.
http://www.millipore.com/immunodetection/id3/weste...
Added: Tue Feb 17 2004, Hits: 11905, Reviews: 0 Write review Cached -
Western Blot
(R&D System)
Provides very nice and general protocol including Western Blot Procedure, immunostaining Procedure (standard and rapid), helpful hints and trouble shooting guide
http://www.rndsystems.com/western_blot_detail_obje...
Added: Tue May 14 2002, Hits: 15292, Reviews: 0 Write review Cached -
Western Blot
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Describes sample preparation, SDS-PAGE, staining, transfer, blotting
http://www.its.caltech.edu/%7Ebjorker/Western_blot...
Added: Wed Aug 14 2002, Hits: 26848, Reviews: 0 Write review -
Western Blotting
(Howell Lab)
Procedures for preparation of cell lysate, gel, electrophoresis, transfer, antibody detection and stripping membranes.
http://cancer.ucsd.edu/howelllab/Western.html
Added: Tue May 14 2002, Hits: 13544, Reviews: 0 Write review Cached -
Anti-Phosphotyrosine Western Blotting
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 540, Reviews: 0 Write review Cached -
MAP Kinase Gel Shift Assay
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 302, Reviews: 0 Write review Cached -
Preparation of Brain Membrane Fractions for Western Blot Analysis
(BD PharMingen)
This is a standard protocol adapted from Rogers et al., 1991.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Tue May 14 2002, Hits: 3818, Reviews: 0 Write review Cached -
Western Blot and Membrane Stripping
(Cowin Lab, New York University Medical Center)
Describes procedures for Western blotting and stripping blot for reblotting.
http://celljunctions.med.nyu.edu/lab/notprivate/me...
Added: Mon Mar 16 2009, Hits: 13890, Reviews: 0 Write review -
Western blot troubleshooting - Blank Film
(J. Rusty Bishop, PhD)
3 hr and 33 minute protocol to troubleshooting the major causes of blank film in a Western blot.
http://westernblot.org/western-blot-troubleshootin...
Added: Wed Aug 17 2011, Hits: 2652, Reviews: 0 Write review -
Western Blot with Platelet Protein
(Viral Immunity & Pathogenesis Group, the Universit� Catholique de Louvain)
Detailed western protocol devoted to detecting paltelet protein but can also be used for general purpose.
http://www.icp.ucl.ac.be/mexp/vipg_plwb.html
Added: Mon Mar 22 2004, Hits: 3275, Reviews: 0 Write review Cached -
Western Blots
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt4/ex4-8...
Added: Tue May 14 2002, Hits: 5285, Reviews: 0 Write review Cached -
Western Blotting
(Corbett Lab)
http://www.biochem.emory.edu/labs/acorbe2/western....
Added: Tue May 14 2002, Hits: 5485, Reviews: 0 Write review -
Western Blotting for Plant Tissue
(Preuss Lab, University of Chicago)
I. Prepare plant tissue samples: II. Pour Acrylamide Gel: III. Gel Electrophoresis: IV. Gel to Filter Blotting: V. Probing the Filter:
http://preuss.bsd.uchicago.edu/protocols/western.h...
Added: Mon Mar 16 2009, Hits: 1665, Reviews: 0 Write review -
Western Blotting Using Chemiluminscence
(Peter J. Hansen Lab)
http://www.animal.ufl.edu/hansen/protocols/blottin...
Added: Tue May 14 2002, Hits: 3044, Reviews: 0 Write review Cached -
Western Blotting with Alkaline Phosphatase Conjugates
(BD PharMingen)
Standard Western protocol describing protein preparation, gel preparation, blotting, blocking...
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Wed Aug 28 2002, Hits: 2209, Reviews: 0 Write review Cached -
Western Blotting with Horseradish Peroxidase Conjugates
(BD Biosciences)
Standard protocol in great detail.
http://www.bdbiosciences.com/support/resources/pro...
Added: Wed Aug 28 2002, Hits: 3006, Reviews: 0 Write review Cached -
Western Blotting with Rabbit Polyclonal Antibodies
(BD Biosciences)
Protocol for western blotting with polyclonal antibodies including troubleshootings.
http://www.bdbiosciences.com/support/resources/pro...
Added: Sun Aug 26 2012, Hits: 946, Reviews: 0 Write review Cached -
Western Procedures
(Gottschling Lab, Fred Hutchinson Cancer Research Center)
Protocols for running protein gel, transfer, staining, stripping and more.
http://labs.fhcrc.org/gottschling/General%20Protoc...
Added: Fri Jul 26 2002, Hits: 4595, Reviews: 0 Write review -
Yeast Lysates for Westerns
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 182, Reviews: 0 Write review Cached
Latest Forum Discussion