Summary: SDS-PAGE stands for Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and is a method used to separate proteins according to their size. Since different proteins with similar molecular weights may migrate differently due to their differences in secondary, tertiary or quaternary structure, SDS, an anionic detergent, is used in SDS-PAGE to reduce proteins to their primary (linearized) structure and coat them with uniform negative charges. Procedures for SDS-PAGE generally involve 1) making a gel and assembling the gel apparatus, 2) mixing protein samples with sample buffer containing SDS and heat the mixture at high temperature, 3) loading samples and running the electrophoresis, 4) fixing and staining the separated proteins.
Introduction to SDS-PAGE(David R. Caprette, Rice University ) Very nice and basic guide to SDS-PAGE gel electrophoresis technique. http://www.ruf.rice.edu/%7Ebioslabs/studies/sds-pa... Added: Sun Sep 29 2002, Hits: 19980, Reviews: 0Write reviewCached
SDS-Page Gel Electrophoresis(School of Chem. and Biochem, Georgia Institutue of Technology) Polyacrylamide gel electrophoresis (PAGE) is probably the most common analytical technique used to separate and characterize proteins. This protocol provides detailed procedures and recipes with illustrations. http://ww2.chemistry.gatech.edu/~lw26/bCourse_Info... Added: Sat Aug 04 2012, Hits: 3606, Reviews: 0Write review
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem) Basic-Native: For Acidic and Neutral proteins (pI <7.0) http://wolfson.huji.ac.il/purification/Protocols/P... Added: Sat Feb 21 2009, Hits: 5370, Reviews: 0Write reviewCached
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem) Acidic-Native: For Basic proteins (pI> 7.0) http://wolfson.huji.ac.il/purification/Protocols/P... Added: Sat Feb 21 2009, Hits: 4211, Reviews: 0Write reviewCached
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem) Tricine: SDS gel for low MW proteins (<25 kDa) http://wolfson.huji.ac.il/purification/Protocols/T... Added: Sat Feb 21 2009, Hits: 1608, Reviews: 0Write reviewCached