Top : Molecular Biology : Protein : Protein Electrophoresis : SDS-PAGE

Summary: SDS-PAGE stands for Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and is a method used to separate proteins according to their size. Since different proteins with similar molecular weights may migrate differently due to their differences in secondary, tertiary or quaternary structure, SDS, an anionic detergent, is used in SDS-PAGE to reduce proteins to their primary (linearized) structure and coat them with uniform negative charges. Procedures for SDS-PAGE generally involve 1) making a gel and assembling the gel apparatus, 2) mixing protein samples with sample buffer containing SDS and heat the mixture at high temperature, 3) loading samples and running the electrophoresis, 4) fixing and staining the separated proteins.
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