Protocols

• Tips and hints for the storage of antibodies (Synaptic Systems)
  http://www.sysy.com/faqs/storage.html
  Added: Tue May 14 2002, Hits: 5049, Reviews: 0  Write review   Cached
• Too Many Bands on Western Blot (Synaptic Systems)
  It is not uncommon that, contrary to the theoretical predictions, several bands are detected. Although it is possible that the antibody is not entirely specific for the protein, other factors may be responsible
  http://www.sysy.com/faqs/western.html
  Added: Tue May 14 2002, Hits: 10634, Reviews: 0  Write review   Cached
• Troubleshooting Tips for Western Blotting (Abcam)
  Tips on common Western blotting problems.
  http://www.abcam.com/ps/pdf/protocols/abcam_troubl...
  Added: Sat Jan 07 2006, Hits: 15975, Reviews: 0  Write review  
• Troubleshooting Tips for Western Immunoblotting (UpState)
  Tips can be used to overcome the most common problems encountered during Western blotting.
  http://www.millipore.com/techpublications/tech1/mc...
  Added: Tue Feb 17 2004, Hits: 18892, Reviews: 0  Write review   Cached
• Western Trouble Shooting (R&D)
  Offers general guide for Western blotting
  http://www.rndsystems.com/tsg_detail_objectname_we...
  Added: Tue May 14 2002, Hits: 15780, Reviews: 0  Write review   Cached

Trouble Shootings and FAQs

• Western Blotting - How to reduce non-specfiic bound signals?
• Western Blotting - High background troubleshooting
• IP-Western Problems - secondary antibody picks up IP antibody
• Tips on Western Blot for Elastin or other Matrix Proteins (very sticky molecule)
• Double Bands in Western Blotting - due to phosphorylation or oxidisation?
• Western blot problem - no chemiluminascence signal
• High background in Western blot
• Western Blot - signal doesn't show up on film
• Too many bands in western blotting
• Western blot - Reversed polarity
• Nonspecific Binding on Western Blot
• Troubles with western blot of MAPK P38
• Troubleshooting membrane protein Western blot
• Western band distorted by 'bubbles'