Subcategories
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Protocols
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Protein Electrophoresis
(Lamond Lab, University of Dundee)
Two protocols are provided: Stripping blots and silver staining.
http://www.lamondlab.com/LTprotGels.htm
Added: Thu May 08 2003, Hits: 6181, Reviews: 0 Write review Cached -
RNase Activity Staining on SDS-PAGE
(Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University)
A detailed procedure for RNase activity staining on SDS-PAGE including recepes.
http://www.bch.msu.edu/pamgreen/rnase.htm
Added: Sat Oct 25 2003, Hits: 565, Reviews: 0 Write review Cached -
Semi-Dry Electrophoretic Blotting of Protein
(Hideaki Shiraishi, Kyoto University)
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
Added: Fri Jan 30 2004, Hits: 1382, Reviews: 0 Write review Cached
Trouble Shootings and FAQs
- SDS-PAGE problem - why I am getting only higher molecular protein bands and none of the middle or low
- SDS- PAGE gel cracks after framing in cellophane
- Problem in getting the protein transferred from polyacrylamide gels after SDS-PAGE
- SDS-PAGE running strangely - poor separation
- Trouble in running SDS-PAGE
- Proteins do not migrate according to their estimate size on SDS-PAGE
- Problem with SDS electrod buffer