Subcategories

Protocols

• Protein Electrophoresis (Lamond Lab, University of Dundee)
  Two protocols are provided: Stripping blots and silver staining.
  http://www.lamondlab.com/LTprotGels.htm
  Added: Thu May 08 2003, Hits: 6181, Reviews: 0  Write review   Cached
• RNase Activity Staining on SDS-PAGE (Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University)
  A detailed procedure for RNase activity staining on SDS-PAGE including recepes.
  http://www.bch.msu.edu/pamgreen/rnase.htm
  Added: Sat Oct 25 2003, Hits: 565, Reviews: 0  Write review   Cached
• Semi-Dry Electrophoretic Blotting of Protein (Hideaki Shiraishi, Kyoto University)
  http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
  Added: Fri Jan 30 2004, Hits: 1382, Reviews: 0  Write review   Cached

Trouble Shootings and FAQs

• SDS-PAGE problem - why I am getting only higher molecular protein bands and none of the middle or low
• SDS- PAGE gel cracks after framing in cellophane
• Problem in getting the protein transferred from polyacrylamide gels after SDS-PAGE
• SDS-PAGE running strangely - poor separation
• Trouble in running SDS-PAGE
• Proteins do not migrate according to their estimate size on SDS-PAGE
• Problem with SDS electrod buffer