Protein (Antibody) Fluorescent Labeling
(Mario Roederer) Gives detailed protocol for conjugation of proteins to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules: http://www.drmr.com/abcon/ Added: Tue May 14 2002, Hits: 1224, Reviews: 0Write reviewCached
(Lamond Lab, University of Dundee) Two protocols are provided: Stripping blots and silver staining. http://www.lamondlab.com/LTprotGels.htm Added: Thu May 08 2003, Hits: 1016, Reviews: 0Write reviewCached
RNase Activity Staining on SDS-PAGE
(Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University) A detailed procedure for RNase activity staining on SDS-PAGE including recepes.
http://ag.udel.edu/plsc/faculty/pamgreen/rnase.htm Added: Sat Oct 25 2003, Hits: 765, Reviews: 0Write reviewCached