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Protocols

• Fluorine labelling of protein (Chen's Lab)
  http://www.biochem.ucl.ac.uk/~chen/protocols/fluoe...
  Added: Tue May 14 2002, Hits: 425, Reviews: 0  Write review  
• Native Gel Electrophoresis of Proteins (Proteinchemist.com)
  http://www.proteinchemist.com/tutorial/natpage.htm...
  Added: Sat Jan 31 2009, Hits: 1933, Reviews: 0  Write review   Cached
• Protein (Antibody) Fluorescent Labeling (Mario Roederer)
  Gives detailed protocol for conjugation of proteins to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules:
  http://www.drmr.com/abcon/
  Added: Tue May 14 2002, Hits: 837, Reviews: 0  Write review   Cached
• Protein Electrophoresis (Lamond Lab, University of Dundee)
  Two protocols are provided: Stripping blots and silver staining.
  http://www.lamondlab.com/LTprotGels.htm
  Added: Thu May 08 2003, Hits: 1016, Reviews: 0  Write review   Cached
• Protein Electrophoresis (electrophoresis.net)
  The basic theory and principles
  http://www.electrophoresis.net/tech_protein.asp
  Added: Sun Jun 06 2004, Hits: 2575, Reviews: 0  Write review  
• RNase Activity Staining on SDS-PAGE (Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University)
  A detailed procedure for RNase activity staining on SDS-PAGE including recepes.
  http://ag.udel.edu/plsc/faculty/pamgreen/rnase.htm
  Added: Sat Oct 25 2003, Hits: 451, Reviews: 0  Write review   Cached
• Semi-Dry Electrophoretic Blotting of Protein (Hideaki Shiraishi, Kyoto University)
  http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
  Added: Fri Jan 30 2004, Hits: 596, Reviews: 0  Write review   Cached