Top : Molecular Biology : Phage : Phage Culture

Protocols

• Assay for Phage Containing the ß- Galactosidase Gene (Donis Keller Lab)
  This assay is used when working with phage vectors carrying the beta-galactosidase gene (often used for immunological screening). If the cloning event disrupts a normally functional copy of the gene in the vector the resulting plaques would appear clear in the assay. If the phages contain a functional beta-galactosidase gene they will form blue rings around their plaques.
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda7....
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• Bacteriophage Plaque lifts (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
  Plaque transfer is typically performed using Amersham gridded, nylon, Hybond-N membranes.
  http://www.nottingham.ac.uk/~mbzspd/methods/Bacter...
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• Calculating Phage Titers (Goldberg Lab)
  http://wheat.pw.usda.gov/~lazo/methods/goldberg/ph...
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• Gel Assay to Determine DNA Content of Phage Lysates (Donis Keller Lab)
  A crude lysate gel assay can be performed to roughly quantitate the DNA in lysates. This is often a valuable time saving step to determine if the phage yield is sufficient to warrant continuing the procedure
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda3....
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• Growth and Purification of 25-100 µg Lambda Clone DNA (Donis Keller Lab)
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda13...
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• Helper Phage Preparation (Vesicle Trafficking)
  http://cmgm.stanford.edu/~vesicle/protocols/helper...
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• Isolation of Phage DNA from Liquid Lysates and CsCl Block Gradients (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
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• Lambda Methods Media and Solutions (Donis Keller Lab)
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda9....
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• Liquid Phage Lysates (Donis Keller Lab)
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda2....
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• Long-Term Lambda Phage Storage (Donis Keller Lab)
  Store phage in 7% DMS0.
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda6....
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• Phage Media Diagram (Goldberg Lab)
  procedures for phage media preparation
  http://wheat.pw.usda.gov/~lazo/methods/goldberg/ph...
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• Phage Plate Stock Lysates (Donis Keller Lab)
  To prepare phage lysates to be used for small or large scale phage DNA preps. This method usually produces lysates with titers of 2-8x10e10 pfu/ml.
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda5....
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• Phage Purification with DE52 (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
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• Picking Single Phage Plaques (Donis Keller Lab)
  For isolating single bacteriophage lambda plaques to be used for making lysates
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda1....
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• Preparation of Phage Lysates (Goldberg Lab)
  Very nice and detailed protocol including recipes for media preparation
  http://wheat.pw.usda.gov/~lazo/methods/goldberg/ph...
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• Streaking Lambda Phages (Donis Keller Lab)
  Phage are streaked onto a medium to obtain an independent isolate prior to preparing a new lysate
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda12...
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• Titering Lambda Phages (Donis Keller Lab)
  This procedure is used to find the number of viable phage particles in a lysate. The phage lysate is serially diluted and ...
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda4....
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• Transfer of Phage DNA from Plaques to Nylon Membrane or Nitrocellulose (Donis Keller Lab)
  To transfer phage DNA to nylon membranes so that a phage library can be screened by hybridization
  http://hg.wustl.edu/hdk_lab_manual/lambda/lambda10...
  Added: Tue May 14 2002, Hits: 425, Reviews: 0  Write review   Cached