June 27 1990
Example of dilutions required:
We assume the lysate has a concentration of 2 x 10e10 pfu/ml. To obtain 200 pfu on the plate we will need a 10e-8 dilution on the plate. To cover the possibility that the titer is nearly 10-fold higher or lower we will also plate out 10e-7 and 10e-9 dilutions (a total of three plates of LBM required).