Top : Immunology : Immunohistochemistry

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• Histological Techniques
• Tissue Microarray

Protocols

• General Instructions for Immunohistochemical Staining (Dako)
  General and very useful guide to immunohistochemistry methods
  http://pri.dako.com/general_instructions_immunohis...
  Added: Fri Apr 03 2009, Hits: 19144, Reviews: 0  Write review  
• Immunohistochemistry on fixed, paraffin-embedded sections (Giorgio Cattoretti, Columbia University)
  Detailed guide with background information. Content includes: Sectioning /Double indirect AP / AP Developing solution / Indirect immunohistochemistry with avidin-biotin and HRP / HRP Developing solution
  http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
  Added: Fri Aug 30 2002, Hits: 10305, Reviews: 0  Write review   Cached
• Introduction to Immunohistochemistry (IHC World)
  General introduction to immunohistochemistry technique including backgroud knowledge, tissue processing, antigen retrieval, and various of IHC methods.
  http://www.ihcworld.com/introduction.htm
  Added: Fri Mar 26 2004, Hits: 13261, Reviews: 0  Write review   Cached
• A semi-permenant mounting medium for immunofluorescence microscopy (House Ear Institute)
  A recipe for Moviol,
  an inexpensive mounting medium that is easy to prepare.
  http://www.hei.org/research/aemi/moviol.htm
  Added: Tue May 14 2002, Hits: 1244, Reviews: 0  Write review   Cached
• BCL6 / PNA Immunohistochemistry (Giorgio Cattoretti, Columbia University)
  Double immunohistochemistry for BCL6 and PNA for Germinal Center detection on fixed, paraffin-embedded sections, including: Indirect immunohistochemistry /HRP Developing solution/Double indirect immunohistochemistry / AP Developing solution
  http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
  Added: Fri Aug 30 2002, Hits: 1349, Reviews: 0  Write review   Cached
• Blocking of unwanted non-specific staining (Giorgio Cattoretti, Columbia University )
  http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
  Added: Fri Aug 30 2002, Hits: 2338, Reviews: 0  Write review   Cached
• Bromodeoxyuridine Immunohistochemistry (Dr. Ornitz's Lab, Washington University Medical School)
  This method utilizes formalin-fixation, paraffin-embedding, and an anti-BrDU mAb that detects BrDU in ssDNA after denaturation and neutralization. Because it has been optimized for midgestational murine lens analysis, it may require further adjustment for other tissues.
  http://molecool.wustl.edu/ornitzlab/proto.BrdU.htm
  Added: Tue May 14 2002, Hits: 2036, Reviews: 0  Write review   Cached
• Dissociated Cell Staining : immunohistochemistry (Mike A. Dyer)
  http://axon.med.harvard.edu/~cepko/protocol/mike/D...
  Added: Tue May 14 2002, Hits: 1104, Reviews: 0  Write review   Cached
• DNAse post-treatment for nuclear antigens (Giorgio Cattoretti, Columbia University)
  DNAse treatment is currently suggested as an unmasking technique for incorporated BrdU nucleotides. However, BrdU detection in dewaxed, fixed tissues with DNAse treatment leads to poorly reproducible results. BrdU instead is readily demonstrated after conventional AR treatment...
  http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
  Added: Fri Aug 30 2002, Hits: 1202, Reviews: 0  Write review   Cached
• Double Immunohistochemistry (Giorgio Cattoretti, Columbia University)
  You should not co-stain in IHC antigens located on the same structure (nucleus, membrane, cytoplasm). That because the success of this double staining procedure is based on the complete development of the first stain, which should mask the structure stained first. Therefore you should be able to use two antibodies raised in the same species. However better results are obtained if two different species are used.
  http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
  Added: Fri Aug 30 2002, Hits: 2587, Reviews: 0  Write review   Cached
• Double in situ Labeling of Embryos (Bier Lab, UCSD)
  
  http://www-bier.ucsd.edu/protocol.html
  Added: Tue May 14 2002, Hits: 246, Reviews: 0  Write review   Cached
• General Immunohistochemistry Protocol (Rosen Lab, Department of Molecular and Cellular Biology, Baylor College of Medicine)
  Step by step guide to immunohistochemistry experiment including deparaffinize and rehydrate, antigen retrieval using sodium citrate, proteinase K and Urea...
  http://public.bcm.tmc.edu/rosenlab/protocols/gener...
  Added: Sat Aug 24 2002, Hits: 9324, Reviews: 0  Write review  
• Immunohistochemical Protocols (The Laboratory of Experimental Pathology (LEP) )
  Immunostaining protocol optimized for individual genes.
  http://dir.niehs.nih.gov/dirlep/immuno.html
  Added: Sat Jul 27 2002, Hits: 5676, Reviews: 0  Write review   Cached
• Immunohistochemical Stain-Alkaline Phosphatase (APAAP) Technique (Nottingham Pathology Lab)
  
  http://www.nottingham.ac.uk/pathology/protocols/ap...
  Added: Tue May 14 2002, Hits: 1906, Reviews: 0  Write review   Cached
• Immunohistochemical Staining for Formalin-Fixed, Paraffin-Embedded Tissues (LEP)
  This staining procedure is for tissues fixed in 10% neutral buffered formalin and embedded in paraffin
  http://dir.niehs.nih.gov/dirlep/protocols/estrogen...
  Added: Tue May 14 2002, Hits: 3731, Reviews: 0  Write review   Cached
• Immunohistochemical Staining Method for Phospho-proteins in Paraffin Sections (BD Biosciences)
  detailed protocol on immunohistochemical staining of Phospho-proteins in paraffin Sections including deparaffinization and re-hydration of tissue slide, antigen retrieval
  http://www.bdbiosciences.com/support/resources/pro...
  Added: Sun Aug 26 2012, Hits: 991, Reviews: 0  Write review   Cached
• Immunohistochemistry (Tyner lab)
  This is a basic immunohistochemistry protocol. Protocols for immunohistochemistry vary widely, due to the differences between antigens and their recognition by antibody. For success it is crucial to try a number of different techniques and compare the results.
  Added: Tue May 14 2002, Reviews: 0  Write review  
• Immunohistochemistry (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
  Step by step procedures with recipes.
  http://www.nottingham.ac.uk/~mbzspd/methods/Immuno...
  Added: Sat Apr 10 2004, Hits: 341, Reviews: 0  Write review   Cached
• Immunohistochemistry - In Situ Hybridization (Hogne Roed Nilsen)
  A detailed look at immunohistochemistry, immunofluorescence and in situ hybridizations.
  http://home.no.net/immuno
  Added: Wed Oct 29 2003, Hits: 2471, Reviews: 0  Write review  
• Immunohistochemistry of Mouse Frozen Tissues (eBioscience)
  http://www.ebioscience.com/ebioscience/appls/IHF.h...
  Added: Tue May 14 2002, Hits: 1960, Reviews: 0  Write review   Cached
• Immunohistochemistry on Drosophila Brains (Vesicle Trafficking, Stanford University)
  http://cmgm.stanford.edu/~vesicle/protocols/immuno...
  Added: Thu Jul 25 2002, Hits: 1851, Reviews: 0  Write review   Cached
• Immunohistochemistry Using the “Milk Method” (Rosen Lab, Department of Molecular and Cellular Biology, Baylor College of Medicine)
  http://public.bcm.tmc.edu/rosenlab/protocols/milkm...
  Added: Wed May 21 2003, Hits: 2088, Reviews: 0  Write review  
• Immunohistochemistry: Preparation and Staining of Paraffin Sections (BD Biosciences)
  Procedures include tissue preparation, fixation (neutral buffered formalin or zinc fixative), sectioning, deparaffinization, rehydration, and immunostaining.
  http://www.bdbiosciences.com/documents/Immuno_Prep...
  Added: Sun Aug 26 2012, Hits: 1355, Reviews: 0  Write review  
• Immunostaining of Paraffin Sections (Laurie Lab, University of Virginia Health Science Center)
  http://people.virginia.edu/~gwl6s/home.html/Method...
  Added: Tue May 14 2002, Hits: 3268, Reviews: 0  Write review   Cached
• Microwave Immunohistochemistry ( Energy Beam Sciences, Inc.)
  Introduction to microwave immunohistochemistry technique and its applicaiton protocol
  http://www.ebsciences.com/papers/mw_immun.htm
  Added: Tue May 14 2002, Hits: 2101, Reviews: 0  Write review   Cached

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