Top : Immunology : Immunohistochemistry : Tips & FAQ
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Protocols
  • Alkaline Phospatase (AP) or Peroxidase (HRP) ? (Giorgio Cattoretti, Columbia University )
    Useful FAQ
    http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
    Added: Fri Aug 30 2002, Hits: 1462, Reviews: 0  Write review   Cached
  • Do I need detergent to permeabilize the cells? (Giorgio Cattoretti, Columbia University )
    FAQ. Once cells are fixed (in suspension, smeared on a slide, cytospun, inside a piece of tissue) they no longer can select intracellular traffic of molecules ...
    http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
    Added: Fri Aug 30 2002, Hits: 1203, Reviews: 0  Write review   Cached
  • Frozen or paraffin sections (Giorgio Cattoretti, Columbia University )
    Which kind of section to use for immunostaining?
    http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
    Added: Fri Aug 30 2002, Hits: 2073, Reviews: 0  Write review   Cached
  • Immuno-FAQ (Giorgio Cattoretti, Columbia University )
    Answers a lot of common questions:
    • Frozen or paraffin sections ?
    • Immunohistochemistry (IHC) or immunofluorescence (IF) ? 
    • Which is the most sensitive method ? IF or IHC ? 
    • Alkaline Phospatase (AP) or Peroxidase (HRP) ? 
    • Which is the most sensitive, HRP or AP? 
    • Which is the most sensitive development system in HRP or AP? 
    • Do I need detergent to permeabilize the cells? 
    • For how long should I incubate a primary antibody? 
    • How stable are the diluted antibodies? 
    • Do I need to freeze my antibody aliquots? 
    • Why my slides do not stain today? 
    • Why only half of the section is stained? 
    • How can I reduce so much background?

    http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
    Added: Fri Aug 30 2002, Hits: 4033, Reviews: 0  Write review   Cached
  • Immunohistochemistry (IHC) or immunofluorescence (IF) ? (Giorgio Cattoretti, Columbia University )
    IHC allows you to see cytologic details and tissue architecture. IF gives quantitative signals ...
    http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
    Added: Fri Aug 30 2002, Hits: 2094, Reviews: 0  Write review   Cached
  • Immunohistochemistry Tips (IHC World)
    Very nicely compiled FAQs on immunohistochemistry.
    http://www.ihcworld.com/technical_tips.htm
    Added: Fri Mar 26 2004, Hits: 2483, Reviews: 0  Write review   Cached
  • Immunohistochemistry Troubleshooting (IHC World)
    Problems and solutions dealing with a variety of IHC problems.
    http://www.ihcworld.com/troubleshooting.htm
    Added: Fri Mar 26 2004, Hits: 1917, Reviews: 0  Write review   Cached
  • Preparation and Storage of Specimens (Giorgio Cattoretti, Columbia University )
    Useful tips for preparation and storage of various of specimens used for immunochemistry.
    http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
    Added: Fri Aug 30 2002, Hits: 2719, Reviews: 0  Write review   Cached
  • Tips for Immunohistochemistry with Fixed Paraffin-Embedded Tissues (Upstate)
    http://www.upstate.com/misc/protocols.q.prot.e.ihc...
    Added: Thu Feb 19 2004, Hits: 1344, Reviews: 0  Write review   Cached
  • Troubleshooting Guide: Immunohistochemistry (R&D Systems)
    Troubleshoots common IHC/ICC issues and provide appropriate experimental actions
    http://www.rndsystems.com/tsg_detail_objectname_im...
    Added: Sat Apr 10 2004, Hits: 1006, Reviews: 0  Write review