Protocols
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Alkaline Phospatase (AP) or Peroxidase (HRP) ?
(Giorgio Cattoretti, Columbia University )
Useful FAQ
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 1620, Reviews: 0 Write review Cached -
Do I need detergent to permeabilize the cells?
(Giorgio Cattoretti, Columbia University )
FAQ. Once cells are fixed (in suspension, smeared on a slide, cytospun, inside a piece of tissue) they no longer can select intracellular traffic of molecules ...
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 1335, Reviews: 0 Write review Cached -
Frozen or paraffin sections
(Giorgio Cattoretti, Columbia University )
Which kind of section to use for immunostaining?
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 2230, Reviews: 0 Write review Cached -
Immuno-FAQ
(Giorgio Cattoretti, Columbia University )
Answers a lot of common questions:- Frozen or paraffin sections ?
- Immunohistochemistry (IHC) or immunofluorescence (IF) ?
- Which is the most sensitive method ? IF or IHC ?
- Alkaline Phospatase (AP) or Peroxidase (HRP) ?
- Which is the most sensitive, HRP or AP?
- Which is the most sensitive development system in HRP or AP?
- Do I need detergent to permeabilize the cells?
- For how long should I incubate a primary antibody?
- How stable are the diluted antibodies?
- Do I need to freeze my antibody aliquots?
- Why my slides do not stain today?
- Why only half of the section is stained?
- How can I reduce so much background?
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 4391, Reviews: 0 Write review Cached -
Immunohistochemistry (IHC) or immunofluorescence (IF) ?
(Giorgio Cattoretti, Columbia University )
IHC allows you to see cytologic details and tissue architecture. IF gives quantitative signals ...
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
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Immunohistochemistry Tips
(IHC World)
Very nicely compiled FAQs on immunohistochemistry.
http://www.ihcworld.com/technical_tips.htm
Added: Fri Mar 26 2004, Hits: 2927, Reviews: 0 Write review Cached -
Immunohistochemistry Troubleshooting
(IHC World)
Problems and solutions dealing with a variety of IHC problems.
http://www.ihcworld.com/troubleshooting.htm
Added: Fri Mar 26 2004, Hits: 2245, Reviews: 0 Write review Cached -
Preparation and Storage of Specimens
(Giorgio Cattoretti, Columbia University )
Useful tips for preparation and storage of various of specimens used for immunochemistry.
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 2963, Reviews: 0 Write review Cached -
Tips for Immunohistochemistry with Fixed Paraffin-Embedded Tissues
(Upstate)
http://www.upstate.com/misc/protocols.q.prot.e.ihc...
Added: Thu Feb 19 2004, Hits: 1569, Reviews: 0 Write review Cached -
Troubleshooting Guide: Immunohistochemistry
(R&D Systems)
Troubleshoots common IHC/ICC issues and provide appropriate experimental actions
http://www.rndsystems.com/tsg_detail_objectname_im...
Added: Sat Apr 10 2004, Hits: 1307, Reviews: 0 Write review