Summary: Antigen Retrieval (AR), developed by Shi et al. in 1991, is a high-temperature heating method to recover the antigenicity of tissue sections that had been masked by formalin fixation. Microwave Oven, Pressure Cooker and Steamer are the most commonly used heating methods. Enzyme digestion by proteases has also been used to retrieve antigen.
- Handbook of Immunochemical Staining Methods: Antigen Retrieval (Dako)
Principal and Technique / Mechanism of Action / Cytology / Target for In Situ Hybridization / Antigen Retrieval and Its Use in Doublestaining
Added: Sat Jul 27 2002, Hits: 5384, Reviews: 0
Antigen Retrieval - Protease Digestion Method
Added: Fri Apr 16 2004, Hits: 2145, Reviews: 0
Antigen Retrieval : for immunohistochemistry
(Mike A. Dyer)
Added: Tue May 14 2002, Hits: 2870, Reviews: 0
Antigen Retrieval for Immunohistochemistry
(Janos Luka Ph.D., Department of Pathology, Eastern Virginia Medical School)
Two methods are presented. One based on microwave treatment, the other on autoclave heating.
Added: Fri Apr 16 2004, Hits: 3242, Reviews: 0
Protocol for Heat-induced Epitope Retrieval (HIER)
(R&D Systems, Inc.)
A detailed protocol for Heat-induced Epitope Retrieval (HIER) is accompanied by a printable schematic.
Added: Wed Jun 02 2010, Hits: 1208, Reviews: 0