Top : Immunology : Immunohistochemistry : Antigen Retrieval

Summary: Antigen Retrieval (AR), developed by Shi et al. in 1991, is a high-temperature heating method to recover the antigenicity of tissue sections that had been masked by formalin fixation. Microwave Oven, Pressure Cooker and Steamer are the most commonly used heating methods. Enzyme digestion by proteases has also been used to retrieve antigen.