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Double Immunohistochemistry
(Giorgio Cattoretti, Columbia University)
You should not co-stain in IHC antigens located on the same structure (nucleus, membrane, cytoplasm). That because the success of this double staining procedure is based on the complete development of the first stain, which should mask the structure stained first. Therefore you should be able to use two antibodies raised in the same species. However better results are obtained if two different species are used.
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 3898, Reviews: 0 Write review Cached -
Double in situ Labeling of Embryos
(Bier Lab, UCSD)
http://www-bier.ucsd.edu/protocol.html
Added: Tue May 14 2002, Hits: 445, Reviews: 0 Write review Cached -
General Immunohistochemistry Protocol
(Rosen Lab, Department of Molecular and Cellular Biology, Baylor College of Medicine)
Step by step guide to immunohistochemistry experiment including deparaffinize and rehydrate, antigen retrieval using sodium citrate, proteinase K and Urea...
http://www.bcm.edu/rosenlab/protocols/generalIHC.p...
Added: Sat Aug 24 2002, Hits: 7770, Reviews: 0 Write review -
IHC Protocol for Free Floating Brain Sections
(Abcam Ltd)
Robust protocol for excellent results
http://www.abcam.com/index.html?rid=189
Added: Mon May 23 2005, Hits: 1159, Reviews: 0 Write review Cached -
Immunohistochemical Protocols
(The Laboratory of Experimental Pathology (LEP) )
Immunostaining protocol optimized for individual genes.
http://dir.niehs.nih.gov/dirlep/immuno.html
Added: Sat Jul 27 2002, Hits: 5098, Reviews: 0 Write review Cached -
Immunohistochemical Special Staining Protocols
(Dako)
Fixation and Tissue Processing Fixation 17; Specimen Processing 21 Staining Methods: Nucleus and Cytoplasm Hematoxylin and Eosin 24; Nucleic Acids 29; Polychromatic Stains 31 Staining Methods:Connective Tissue, Muscle Fibers and Lipids Connective Tissue 34; Reticular Fibers 35; Basement Membranes 36; Elastic Fibers 38; Mast Cells 39; Muscle 41; Trichrome Stains 41; PTAH 44; Modified Russel-Movat Pentachrome 45; Lipids 46 Staining Methods: Carbohydrates and Amyloid Carbohydrates 48; Amyloid 48; Periodic Acid-Schiff (PAS) 49; PAS with Diastase 51; Mucicarmine 52; Alcian Blue 53; Alcian Blue/PAS 55; Colloidal Iron 56; Congo Red 57 Staining Methods: Microorganisms Bacteria 60; Fungi 61; Protozoans 61; Gram Stain 62; Acid-Fast Bacteria Stain 63; Grocott’s Methenamine Silver Stain 64; Warthin-Starry Stain 65; Alcian Yellow/Toluidine Blue Stain 66; Giemsa Stain 68; Periodic Acid-Schiff-Green Stain 69 Staining Methods: Pigments and Minerals Melanin 71; Hemosiderin 73; Lipofuscin 74; Urates 75; Bile 76; Calcium 77; Copper 78 Staining Methods: Nervous System Cresyl Violet Stain 80; Bielschowsky Method 81; Glial Cells 82; Myelin 84
http://pri.dako.com/08066_guide_to_special_stains....
Added: Sun Mar 02 2008, Hits: 831, Reviews: 0 Write review -
Immunohistochemical Stain-Alkaline Phosphatase (APAAP) Technique
(Nottingham Pathology Lab)
http://www.nottingham.ac.uk/pathology/protocols/ap...
Added: Tue May 14 2002, Hits: 1484, Reviews: 0 Write review Cached -
Immunohistochemical Staining for Formalin-Fixed, Paraffin-Embedded Tissues
(LEP)
This staining procedure is for tissues fixed in 10% neutral buffered formalin and embedded in paraffin
http://dir.niehs.nih.gov/dirlep/protocols/estrogen...
Added: Tue May 14 2002, Hits: 2046, Reviews: 0 Write review Cached - Immunohistochemistry (Tyner lab)
This is a basic immunohistochemistry protocol. Protocols for immunohistochemistry vary widely, due to the differences between antigens and their recognition by antibody. For success it is crucial to try a number of different techniques and compare the results.
Added: Tue May 14 2002, Reviews: 0 Write review -
Immunohistochemistry
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Step by step procedures with recipes.
http://www.nottingham.ac.uk/~mbzspd/methods/Immuno...
Added: Sat Apr 10 2004, Hits: 1551, Reviews: 0 Write review Cached