Overview: Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues and consists of the following steps: 1)primary antibody binds to specific antigen; 2) antibody-antigen complex is bound by a secondary, enzyme-conjugated, antibody; 3) in the presence of substrate and chromogen, the enzyme forms a colored deposit at the sites of antibody-antigen binding.
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Protocols
- Immunohistochemistry on fixed, paraffin-embedded sections (Giorgio Cattoretti, Columbia University)
Detailed guide with background information. Content includes: Sectioning /Double indirect AP / AP Developing solution / Indirect immunohistochemistry with avidin-biotin and HRP / HRP Developing solution
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 14785, Reviews: 0 Write review Cached - Introduction to Immunohistochemistry (IHC World)
General introduction to immunohistochemistry technique including backgroud knowledge, tissue processing, antigen retrieval, and various of IHC methods.
http://www.ihcworld.com/introduction.htm
Added: Fri Mar 26 2004, Hits: 19855, Reviews: 0 Write review Cached - Protocol for Immunohistochemistry on Mouse Spine (Azadeh Nasrazadani, The Van Den Berg Lab, UT Austin)
Bone immunohistochemistry is a relatively tricky procedure. This protocol ensures that mouse spine tissue will stay on the slide throughout the process.
Added: Thu Aug 02 2007, Reviews: 0 Write review -
A semi-permenant mounting medium for immunofluorescence microscopy
(House Ear Institute)
A recipe for Moviol,
an inexpensive mounting medium that is easy to prepare.
http://www.hei.org/research/aemi/moviol.htm
Added: Tue May 14 2002, Hits: 1928, Reviews: 0 Write review Cached -
BCL6 / PNA Immunohistochemistry
(Giorgio Cattoretti, Columbia University)
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection on fixed, paraffin-embedded sections, including: Indirect immunohistochemistry /HRP Developing solution/Double indirect immunohistochemistry / AP Developing solution
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 1524, Reviews: 0 Write review Cached -
Blocking of unwanted non-specific staining
(Giorgio Cattoretti, Columbia University )
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 3601, Reviews: 0 Write review Cached -
Bromodeoxyuridine (BrdU) Staining
(Abcam Ltd)
Identification of labelled cells can be applied both in vitro and in vivo to produce a nuclear staining pattern.
http://www.abcam.com/index.html?rid=10203
Added: Mon May 23 2005, Hits: 3688, Reviews: 0 Write review Cached -
Bromodeoxyuridine Immunohistochemistry
(Dr. Ornitz's Lab, Washington University Medical School)
This method utilizes formalin-fixation, paraffin-embedding, and an anti-BrDU mAb that detects BrDU in ssDNA after denaturation and neutralization. Because it has been optimized for midgestational murine lens analysis, it may require further adjustment for other tissues.
http://molecool.wustl.edu/ornitzlab/PDFs/Protocols...
Added: Tue May 14 2002, Hits: 3495, Reviews: 0 Write review Cached -
Dissociated Cell Staining : immunohistochemistry
(Mike A. Dyer)
http://axon.med.harvard.edu/~cepko/protocol/mike/D...
Added: Tue May 14 2002, Hits: 1968, Reviews: 0 Write review Cached -
DNAse post-treatment for nuclear antigens
(Giorgio Cattoretti, Columbia University)
DNAse treatment is currently suggested as an unmasking technique for incorporated BrdU nucleotides. However, BrdU detection in dewaxed, fixed tissues with DNAse treatment leads to poorly reproducible results. BrdU instead is readily demonstrated after conventional AR treatment...
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 1525, Reviews: 0 Write review Cached