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Protocols
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AP Assay and FGF Binding
(Dr. Ornitz's Lab, Washington University Medical School)
FGFR-AP-FGF Binding Assay, PCR Assays for Knockout Mice, RNase Protection Assay:The bound receptor was quantified by measuring the initial rate of substrate hydrolysis at 405 nm and same amount of proteins was used in the binding assays...
http://molecool.wustl.edu/ornitzlab/PDFs/Protocols...
Added: Tue May 14 2002, Hits: 524, Reviews: 0 Write review Cached -
Combined LacZ staining and in situ hybridization
(Practical Approach Online, Oxford University Press)
Mouse embryos staining, including: in vitro transcription of riboprobe, embryo fixation and permeabilization, hybridization and post-hybridization washes, immunodetection and development of signal.
http://www.oup.co.uk/pdf/pas/13-3-9.pdf
Added: Wed Sep 04 2002, Hits: 575, Reviews: 0 Write review -
DNA Extraction from Tail Biopsies
(Jackson Labs)
Phenol:chloroform extraction method
http://www.jax.org/imr/tail_phenol.html
Added: Tue May 14 2002, Hits: 842, Reviews: 0 Write review Cached -
DNA Preparation from Mouse Tails for Southern Blotting
(Dr. Ornitz's Lab, Washington University Medical School)
Avoid Shearing of the DNA by limiting mixing
to inversion and gentle shaking. Vortexing of samples is not required to perform the following protocols. Avoid contamination by rigorously cleaning pipetman and using autoclaved tips during manipulation of genomic DNA and reagents to be used with genomic DNA...
http://molecool.wustl.edu/ornitzlab/PDFs/Protocols...
Added: Tue May 14 2002, Hits: 794, Reviews: 0 Write review Cached -
DNA Purification for PCR only from yolk sacs, toes, or 2 mm Tail
(U. of Virginia Transgenic Mouse Core Facility)
http://www.med.virginia.edu/medicine/inter-dis/tra...
Added: Tue May 14 2002, Hits: 362, Reviews: 0 Write review Cached -
Embedding, sectioning and counterstaining of LacZ embryos
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-3-13.pdf
Added: Wed Sep 04 2002, Hits: 416, Reviews: 0 Write review -
G-banding for analysing mouse chromosomal rearrangements
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-6a-2.pdf
Added: Wed Sep 04 2002, Hits: 882, Reviews: 0 Write review -
Genomic Southerns of DNA from Tail Clips
(Transgenic and ES Cell Services at OSU)
http://www.med.ohio-state.edu/tafhome/webpages/pro...
Added: Tue May 14 2002, Hits: 327, Reviews: 0 Write review Cached -
Genotyping Transgenic Mice by PCR
(University of Michigan Transgenic Animal Model Core)
Test mice for the presence of the transgene by PCR. It is provided for those investigators who are unfamiliar with mouse genotyping and who need to establish a genotyping method.
http://www.med.umich.edu/tamc/pcr.html
Added: Tue May 14 2002, Hits: 1886, Reviews: 0 Write review Cached -
Isolation of DNA from Mouse Tail Biopsies
(University of Michigan Transgenic Animal Model Core)
http://www.med.umich.edu/tamc/tDNA.html
Added: Tue May 14 2002, Hits: 716, Reviews: 0 Write review Cached