Related Categories:

• Whole Mount Staining

Protocols

• AP Assay and FGF Binding (Dr. Ornitz's Lab, Washington University Medical School)
  FGFR-AP-FGF Binding Assay, PCR Assays for Knockout Mice, RNase Protection Assay:The bound receptor was quantified by measuring the initial rate of substrate hydrolysis at 405 nm and same amount of proteins was used in the binding assays...
  https://molecool.wustl.edu/Ornitz/proto.assays.htm
  Added: Tue May 14 2002, Hits: 74, Reviews: 0  Write review   Cached
• Combined LacZ staining and in situ hybridization (Practical Approach Online, Oxford University Press)
  Mouse embryos staining, including: in vitro transcription of riboprobe, embryo fixation and permeabilization, hybridization and post-hybridization washes, immunodetection and development of signal.
  https://www.oup.co.uk/pdf/pas/13-3-9.pdf
  Added: Wed Sep 04 2002, Hits: 122, Reviews: 0  Write review  
• DNA Extraction from Tail Biopsies (Jackson Labs)
  Phenol:chloroform extraction method
  https://www.jax.org/imr/tail_phenol.html
  Added: Tue May 14 2002, Hits: 146, Reviews: 0  Write review  
• DNA preparation from mouse tails (Dr. Ornitz's Lab, Washington University Medical School)
  Avoid Shearing of the DNA by limiting mixing
  to inversion and gentle shaking. Vortexing of samples is not required to perform the following protocols. Avoid contamination by rigorously cleaning pipetman and using autoclaved tips during manipulation of genomic DNA and reagents to be used with genomic DNA...
  https://molecool.wustl.edu/Ornitz/proto.tailprep.ht...
  Added: Tue May 14 2002, Hits: 174, Reviews: 0  Write review   Cached
• DNA Purification for PCR only from yolk sacs, toes, or 2 mm Tail (U. of Virginia Transgenic Mouse Core Facility)
  https://www.med.virginia.edu/medicine/inter-dis/tra...
  Added: Tue May 14 2002, Hits: 78, Reviews: 0  Write review   Cached
• Embedding, sectioning and counterstaining of LacZ embryos (Practical Approach Online, Oxford University Press)
  https://www.oup.co.uk/pdf/pas/13-3-13.pdf
  Added: Wed Sep 04 2002, Hits: 71, Reviews: 0  Write review  
• G-banding for analysing mouse chromosomal rearrangements (Practical Approach Online, Oxford University Press)
  https://www.oup.co.uk/pdf/pas/13-6a-2.pdf
  Added: Wed Sep 04 2002, Hits: 159, Reviews: 0  Write review  
• Genomic Southerns of DNA from Tail Clips (Transgenic and ES Cell Services at OSU)
  https://www.med.ohio-state.edu/tafhome/webpages/pro...
  Added: Tue May 14 2002, Hits: 100, Reviews: 0  Write review   Cached
• Genotyping Transgenic Mice by PCR (University of Michigan Transgenic Animal Model Core)
  Test mice for the presence of the transgene by PCR. It is provided for those investigators who are unfamiliar with mouse genotyping and who need to establish a genotyping method.
  https://www.med.umich.edu/tamc/pcr.html
  Added: Tue May 14 2002, Hits: 279, Reviews: 0  Write review   Cached
• Isolation of DNA from Mouse Tail Biopsies (University of Michigan Transgenic Animal Model Core)
  https://www.med.umich.edu/tamc/tDNA.html
  Added: Tue May 14 2002, Hits: 92, Reviews: 0  Write review   Cached
• Isolation of DNA from Mouse Tail Biopsies (University of Michigan Transgenic Animal Model Core)
  https://www.med.umich.edu/tamc/tDNA.html
  Added: Tue May 14 2002, Hits: 62, Reviews: 0  Write review   Cached
• LacZ Cell Staining and β-galactosidase Embryo Staining (Dr. Ornitz's Lab, Washington University Medical School)
  https://molecool.wustl.edu/Ornitz/proto.LacZ.htm
  Added: Tue May 14 2002, Hits: 112, Reviews: 0  Write review   Cached
• Mitotic chromosomes from mouse peripheral blood (Practical Approach Online, Oxford University Press)
  https://www.oup.co.uk/pdf/pas/13-6a-1.pdf
  Added: Wed Sep 04 2002, Hits: 120, Reviews: 0  Write review  
• Mouse Tail (or organ) Biopsy DNA Extraction (The Cancer Center at Washington University)
  https://medicine.wustl.edu/~escore/htmldocs/tailpre...
  Added: Tue May 14 2002, Hits: 77, Reviews: 0  Write review   Cached
• Mouse Tail Digestion Procedure (Pharmacological Sciences Consortium Core)
  https://transgenic.cwru.edu/tail.htm
  Added: Tue May 14 2002, Hits: 78, Reviews: 0  Write review   Cached
• Non-organic Isolation of Tail DNA (Jackson Labs)
  Using tail tissue directly for PCR after proteinase K digestion
  https://www.jax.org/imr/tail_nonorg.html
  Added: Tue May 14 2002, Hits: 102, Reviews: 1 Read reviews  Write review  
• PCR Analysis of Tail DNA (University of Michigan, Transgenic Animal Model Core)
  https://www.med.umich.edu/tamc/pcr.html
  Added: Tue May 14 2002, Hits: 90, Reviews: 0  Write review   Cached
• PCR of DNA from Ear Clip Tissue (Transgenic and ES Cell Services at OSU)
  https://www.med.ohio-state.edu/tafhome/webpages/pro...
  Added: Tue May 14 2002, Hits: 55, Reviews: 0  Write review   Cached
• Preparation of Copy Standards for PCR Genotyping (University of Michigan Transgenic Animal Model Core)
  The purpose of spiking tail DNA with a known amount of transgene DNA is to produce copy standards. These are used to verify the sensitivty of your PCR genotyping assay and to establish transgene copy number in Southern blot analysis.
  https://www.med.umich.edu/tamc/spike.html
  Added: Tue May 14 2002, Hits: 120, Reviews: 0  Write review   Cached
• Preparation of Mouse Tail DNA for Dot Blots or PCR  (Eric Mercer)
  These procedures were originally devised in Richard Palmiter's lab for use with tail dots (DNA spotted onto a nitrocellulose filter and probed for a transgene; quicker than doing southerns), and subsequently modified for PCR preps.
  https://www.muridae.com/wmc/docs/tailDNA_prep.html
  Added: Tue May 14 2002, Hits: 92, Reviews: 0  Write review   Cached
• RNA Whole Mount In Situ Hybridization (Cepko/Tabin Lab)
  protocol for both mouse and chick embryos
  https://axon.med.harvard.edu/~cepko/protocol/ctlab/...
  Added: Tue May 14 2002, Hits: 84, Reviews: 0  Write review   Cached
• Tail Preps: DNA Isolation From Mouse Tails Without Phenol (Hedrick Lab, UCSD Cancer Center)
  DNA isolation for Southern analysis
  https://hedricklab.ucsd.edu/Protocol/TailPreps.html
  Added: Tue Dec 10 2002, Hits: 369, Reviews: 0  Write review   Cached
• Whole Mount Human Placental Alkaline Phosphatase Staining (Nagy Lab)
  https://www.mshri.on.ca/nagy/Protocols/APwmstain.ht...
  Added: Tue May 14 2002, Hits: 42, Reviews: 0  Write review   Cached
• Whole Mount Immunoflurescentstaining (Nagy Lab)
  https://www.mshri.on.ca/nagy/Protocols/wmimmun.htm
  Added: Tue May 14 2002, Hits: 46, Reviews: 0  Write review   Cached
• Whole Mount LacZ Staining (Nagy Lab)
  https://www.mshri.on.ca/nagy/Protocols/lacZ.htm
  Added: Tue May 14 2002, Hits: 82, Reviews: 0  Write review   Cached

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