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Protocols
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whole mount preparation and lacZ staining in the mammary gland
(Laboratory of Genetics & Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH)
The bacterial beta-galactosidase gene lacZ is frequently used as a reporter gene. The expression of transgenic constructs can be monitored by histochemistry with the chromogenic substrate X-gal. This allows precise cellular localization of gene activity.
https://mammary.nih.gov/tools/histological/Histolog...
Added: Thu Jul 25 2002, Hits: 98, Reviews: 0
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Whole Mount RNA in situ Hybridization
(Nagy Lab)
RNA probe labeling and purification, embryo preparation and more...
https://www.mshri.on.ca/nagy/Protocols/wmrna.htm
Added: Tue May 14 2002, Hits: 74, Reviews: 0
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X-gal & AP Staining Protocol
(Cepko/Tabin Lab)
Very detailed protocol with background information and several applications.
https://axon.med.harvard.edu/~cepko/protocol/xgalpl...
Added: Tue May 14 2002, Hits: 110, Reviews: 0
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Yolk Sac DNA Preparationor Southern or PCR
(U. of Virginia Transgenic Mouse Core Facility)
https://www.med.virginia.edu/medicine/inter-dis/tra...
Added: Tue May 14 2002, Hits: 29, Reviews: 0
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