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Protocols
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Derivation of Human Embryonic Stem Cells by Immunosurgery (Journal of Visualized Experiments )
This video protocol describes procedrues for estabilishing human ES cells including preparation of plates for immunosurgery, preparing capillary pipettes, immunosurgery to isolate Inner Cell Mass (ICM), development of human ES cell outgrowth, passaging outgrowth. A text protocol is also provided.
http://www.jove.com/index/Details.stp?ID=574
Added: Mon Feb 25 2008, Hits: 185, Reviews: 0 Write review Cached - ES Cell Culture and Manipulation (The Cancer Center at Washington University)
A collection of protocols related to ES cell culture, identification, feeder cell preparation and more.
http://escore.im.wustl.edu/htmldocs/protocol.htm
Added: Mon Mar 05 2007, Hits: 1240, Reviews: 0 Write review Cached - ES Cell Culture Protocols (Chemicon)
This page decribes protocols for isolation and culture of primary mouse embryonic fibroblasts, feeder cell irradiation and freezing, ES cell culture and electroporation, cloning picking, DNA isolation, karyotyping analysis of ES cells, and reagents.
http://www.esgro-lif.com/esgro/protocols.htm
Added: Wed Mar 22 2006, Hits: 3002, Reviews: 0 Write review Cached - General ES Cell Protocols/Starting Out With ES Cells (Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
ES cell thawing and preservation, maintaining.
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/e...
Added: Sun Jul 28 2002, Hits: 3482, Reviews: 0 Write review Cached -
Aggregation of ES Cells and Eight Cell Stage embryos
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 667, Reviews: 0 Write review Cached -
Aggregation of ES cells with mouse morula-stage embryos
(Practical Approach Online, Oxford University Press)
Describes: Preparation of aggregation wells, ES cell preparation, and embryo preparation and aggregation
http://www.oup.co.uk/pdf/pas/13-10-6.pdf
Added: Mon Sep 02 2002, Hits: 952, Reviews: 0 Write review -
Blastocyst Transfer
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 383, Reviews: 0 Write review Cached -
Cell Culture from Whole Mice Embryo
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
Obtain ES cell from mice embryo for culture
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 787, Reviews: 0 Write review Cached -
Culture of Human Embryonic Stem Cells (hESC)
(NIH Stem Cell Unit)
A simplified protcol for growing ES cells from different sources.
http://stemcells.nih.gov/research/NIHresearch/scun...
Added: Fri Oct 07 2005, Hits: 1223, Reviews: 0 Write review Cached -
ES / MEF cell culture and electroporation of targeting construct
(Gary Brown)
http://ourworld.compuserve.com/homepages/TheBroons...
Added: Tue May 14 2002, Hits: 1323, Reviews: 0 Write review Cached