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Protocols
- Culture and Maintenance of Human Embryonic Stem Cells
(JoVE)
Human embryonic stem (hES) cells must be monitored and cared for in order to maintain healthy, undifferentiated cultures. At minimum, the cultures must be fed every day by performing a complete medium change to replenish lost nutrients and to keep the cultures free of unwanted differentiation factors. Although a small amount of differentiation is normal and expected in stem cell cultures, the culture should be routinely cleaned up by manually removing, or "picking" differentiated areas...
http://www.jove.com/details.php?id=1427
Added: Thu Apr 14 2011, Hits: 1186, Reviews: 0 Write review - General ES Cell Protocols/Starting Out With ES Cells (Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
ES cell thawing and preservation, maintaining.
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/e...
Added: Sun Jul 28 2002, Hits: 1978, Reviews: 0 Write review Cached - Guidelines for Culturing ES and MEF Feeder Cells (Transgenic Research Center, Stanford University)
Routine maintenance of ES cells, culturing feeder cells, cell freezing and thawing.
http://med.stanford.edu/transgenic/esmeflif.html
Added: Wed Jan 28 2009, Hits: 1469, Reviews: 0 Write review -
Aggregation of ES Cells and Eight Cell Stage embryos
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 221, Reviews: 0 Write review Cached -
Aggregation of ES cells with mouse morula-stage embryos
(Practical Approach Online, Oxford University Press)
Describes: Preparation of aggregation wells, ES cell preparation, and embryo preparation and aggregation
http://www.oup.co.uk/pdf/pas/13-10-6.pdf
Added: Mon Sep 02 2002, Hits: 824, Reviews: 0 Write review -
b-Galactosidase Color Assay for Cultured Cells
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/b...
Added: Sun Jul 28 2002, Hits: 757, Reviews: 0 Write review Cached -
Blastocyst Transfer
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 158, Reviews: 0 Write review Cached -
Cell Culture from Whole Mice Embryo
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
Obtain ES cell from mice embryo for culture
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 274, Reviews: 0 Write review Cached -
Cortical Stem Cell Culture Expansion Protocol
(R&D System)
http://www.rndsystems.com/stem_cell_protocol_detai...
Added: Wed Feb 04 2009, Hits: 667, Reviews: 0 Write review Cached -
Culturing Embryonic Stem Cells on a Feeder Cell Layer
(R&D Systems)
A protocol for culturing BG01V embryonic stem cells on a feeder cell layer of irradiated fibroblasts.
http://www.rndsystems.com/stem_cell_protocol_detai...
Added: Fri Mar 20 2009, Hits: 824, Reviews: 0 Write review Cached -
Electroporation of ES Cells and Isolation of H/R Clones
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 179, Reviews: 0 Write review Cached -
ES-cell injection into mouse blastocysts
(Practical Approach Online, Oxford University Press)
Step by step guide for ES cell microinjection, required reagents and equipment.
http://www.oup.co.uk/pdf/pas/13-10-4.pdf
Added: Wed Sep 04 2002, Hits: 797, Reviews: 0 Write review -
Feeder-Dependent Culture of hESCs and hiPSCs
(NIH Stem Cell Information)
http://stemcells.nih.gov/research/nihresearch/scun...
Added: Mon May 20 2013, Hits: 711, Reviews: 0 Write review Cached -
Injection of ES cells into morulae
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-10-5.pdf
Added: Wed Sep 04 2002, Hits: 659, Reviews: 0 Write review -
Normaski Optics
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 103, Reviews: 0 Write review Cached -
Preparation of RNA from Cultured ES Cells
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/r...
Added: Sun Jul 28 2002, Hits: 699, Reviews: 0 Write review Cached -
Production of Completely ES Cell-Derived Fetuses by Aggragation with Tetraploid Embryos
(Nagy Lab)
http://www.mshri.on.ca/nagy/Tetraploid/Tetra.htm
Added: Tue May 14 2002, Hits: 294, Reviews: 0 Write review Cached -
Production of ES Cell Chimeras by Aggregation wtih Eight-Cell Stage Embryos
(Nagy Lab)
To produce completely ES cell-derived embryos a clump of ES cells is sandwiched between two tetraploid embryos. For ES cell aggregation chimeras with diploid embryos, one embryo is aggregated with a clump of ES cells. Even in this case, the ES cell internalization is efficient enough to achieve a high level of ES cell contribution.
http://www.mshri.on.ca/nagy/diploid/diploid.htm
Added: Tue May 14 2002, Hits: 344, Reviews: 0 Write review Cached -
Routine Splitting and Freezing of Cells
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 157, Reviews: 0 Write review Cached -
Stem Cell Culture
(Labome)
Detailed stem cell culture protocol, including reagents and suppliers, step,, and notes.
http://www.labome.com/about/stem-cell-protocol.htm...
Added: Fri Nov 05 2010, Hits: 802, Reviews: 0 Write review -
Vasectomy of Male Mice
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 103, Reviews: 0 Write review Cached