Top : Cell Biology : Stem Cells : Embryonic Stem (ES) Cell Culture : Mouse Embryonic Fibroblast (MEF) Feeder Cell Preparation

Summary: Mouse Embryonic Fibroblast (MEF) cells are required to support the growth of undifferentiated mouse or human ES cells, iPS cells, thus they are also called feeder cells. MEF cells are isolated from mouse embryos and are used at their early passages. Before MEF cells are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing. Protocols in this section cover MEF isolation, freezing and thawing, and treatment.