Summary: Mouse Embryonic Fibroblast (MEF) cells are required to support the growth of undifferentiated mouse or human ES cells, iPS cells, thus they are also called feeder cells. MEF cells are isolated from mouse embryos and are used at their early passages. Before MEF cells are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing. Protocols in this section cover MEF isolation, freezing and thawing, and treatment.
Subcategories
Protocols
- Harvesting Mouse Embryonic Fibroblasts from Murine Embryos
(Thomson Lab, University of Wisconsin)
http://ink.primate.wisc.edu/~thomson/pdfs/harvesti...
Added: Wed Mar 25 2009, Hits: 3651, Reviews: 0 Write review - MEF Cell Isolation, Culture, Freezing, Thawing and Mitomycin C Treatment (Himmelbauer Lab, Max-Planck-Institute for Molecular Genetics)
Procedures for isolation of MEF cells, culturing, freezing, thawing and mitomycin C treatment.
http://www.molgen.mpg.de/~rodent/MEF_protocol.pdf
Added: Wed Mar 25 2009, Hits: 5181, Reviews: 0 Write review -
Culturing Mouse Embryonic Fibroblasts
(Fero Lab, Fred Hutchinson Cancer Research Center)
This protocol describes the isolation of primary MEFs and passage according to the 3T3 protocol.
http://labs.fhcrc.org/fero/Protocols/MEFs.html
Added: Sat Jan 31 2009, Hits: 3667, Reviews: 0 Write review Cached -
Feeder Preparation by Gamma Irration
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/g...
Added: Sun Jul 28 2002, Hits: 1298, Reviews: 0 Write review Cached - Freezing and Thawing of MEFs
This protocol is very useful to the researchers working on primary mouse embryonic fibrobasts and gene cell lines
Added: Mon Feb 02 2009, Reviews: 0 Write review -
Gamma Irradiation of PMEF'S
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 111, Reviews: 0 Write review Cached -
Isolation of Primary Fibroblasts from Mouse Embryos
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/p...
Added: Sun Jul 28 2002, Hits: 1644, Reviews: 0 Write review Cached -
Isolation of Primary Mouse Embryo Fibroblasts
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 233, Reviews: 0 Write review Cached -
Isolation of Primary Mouse Embryo Fibroblasts
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 224, Reviews: 0 Write review Cached -
PMEF Feeder Layer Concentration
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 120, Reviews: 0 Write review Cached -
Preparation of Feeder Layers And SNL Stocks
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/f...
Added: Sun Jul 28 2002, Hits: 783, Reviews: 0 Write review Cached -
Preparation of Murine Embryonic Fibroblasts
(Gary Brown)
http://ourworld.compuserve.com/homepages/TheBroons...
Added: Tue May 14 2002, Hits: 579, Reviews: 0 Write review Cached -
Preparing 48-Well Plate Feeders
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/4...
Added: Sun Jul 28 2002, Hits: 626, Reviews: 0 Write review Cached