Protocols
- ES Cell Culture and Manipulation ( The Chisholm Lab, Northwestern University Medical School)
Detailed protocol for ES cell manipulation, including Media, plating ES cell, Splitting ES cells, freezing ES cells, transformation of ES cells, expansion of ES cell clones and more...
http://dicty.cmb.nwu.edu/Chis_lab/Lab%20Manual/es_...
Added: Tue May 14 2002, Hits: 888, Reviews: 0 Write review Cached - ES cell culture protocol
(The Wellcome Trust Sanger Institute)
This protocol describes procedures including ES cell thawing, passaging, expanding and freezing.
http://www.sanger.ac.uk/PostGenomics/genetrap/prot...
Added: Wed Jan 28 2009, Hits: 3023, Reviews: 0 Write review - Mouse Embryonic Stem (ES) Cell Culture - Basic Procedures (Himmelbauer Lab, Max-Planck-Institute for Molecular Genetics)
This protocol presents something like a brief "best of" collection from various sources plus some or the author's own experiences.
http://www.molgen.mpg.de/~rodent/ES_protocol.pdf
Added: Wed Mar 25 2009, Hits: 2835, Reviews: 0 Write review -
Culturing ES Cells
(Lahn's Lab, University of Chicago)
Prepare gelatin-coated flasks and passing ES cells
http://hominid.uchicago.edu/ProtocolPDFs/Culturing...
Added: Fri Mar 06 2009, Hits: 1621, Reviews: 0 Write review -
ES / MEF cell culture and electroporation of targeting construct
(Gary Brown)
http://ourworld.compuserve.com/homepages/TheBroons...
Added: Tue May 14 2002, Hits: 519, Reviews: 0 Write review Cached -
ES Cell Culture and Manipulation
(The Cancer Center at Washington University)
http://medicine.wustl.edu/~escore/htmldocs/protoco...
Added: Tue May 14 2002, Hits: 615, Reviews: 0 Write review Cached -
ES Stem Cell Troubleshooting Guide
(Millipore)
http://www.millipore.com/publications.nsf/a73664f9...
Added: Wed Feb 04 2009, Hits: 1215, Reviews: 0 Write review -
Picking ES Cell Colonies and Transferring Them to 24-Well Culture Dishes
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/p...
Added: Sun Jul 28 2002, Hits: 743, Reviews: 0 Write review Cached -
Routine Culturing of ES Cells
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 203, Reviews: 0 Write review Cached