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Protocols

• Human Embryonic Stem (ES) Cell Protocols (Thomson Lab, University of Wisconsin)
  The following procedures are included:
  • Media and reagents
  • Thawing ES Cells
  • Splitting ES Cells on MEF's (stem cell photos)
  • Freezing ES Cells
  • Matrigel Aliquoting and Plating
  • Splitting ES Cells on Matrigel
  • Embryonic Bodies
  • General Notes on ES Cells
  
  http://ink.primate.wisc.edu/~thomson/protocol.html
  Added: Thu Jan 29 2009, Hits: 1056, Reviews: 0  Write review   Cached
• Culture of Human Embryonic Stem Cells (hESC) (NIH Stem Cell Information)
  Methods for culturing human ES cells with medium recipes.
  http://stemcells.nih.gov/research/NIHresearch/scun...
  Added: Thu Jan 29 2009, Hits: 999, Reviews: 0  Write review   Cached
• Culturing BG01V Human Embryonic Stem Cells with MEF-conditioned Medium (R&D System)
  Procedure for Culturing BG01V Human Embryonic Stem Cells on Irradiated Mouse Embryonic Fibroblasts (iMEF)
  http://www.rndsystems.com/stem_cell_protocol_detai...
  Added: Thu Jan 29 2009, Hits: 508, Reviews: 0  Write review   Cached
• Methods for Expansion of Human Embryonic Stem Cells (Stem Cell)
  This article describes both mechanical and enzymatic transfer methods for human embryonic stem cells (hESCs) depending on experimental purpose.
  http://stemcells.alphamedpress.org/cgi/content/ful...
  Added: Wed Jan 28 2009, Hits: 727, Reviews: 0  Write review   Cached