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Cell Biology Protocols
(Lamond Lab, University of Dundee)
The Cell section of Lamond Lab's site hosts the following protocols:
- Establishing stable cell lines
- Heterokaryons
- FRET measurement (CFP/YFP)
- Lysing cells to release nuclear and cytoplasmic proteins
- Preparing nuclear extracts
http://www.lamondlab.com/LTprotCells.htm
Added: Thu May 08 2003, Hits: 3755, Reviews: 0 Write review Cached -
L-selectin-PNAd Interactions under Flow Conditions
(Springer Lab, Harvard University)
The main purpose of the flow chamber assay is to visualize and measure interactions between flowing cells expressing a given adhesion molecule on their surface, and their receptor, either directly coated on the flow chamber lower wall or expressed on a cell monolayer. This protocol focuses on the interactions between L-selectin expressed on neutrophils and PNAd coated onto the plastic surface.
http://www.cbrinstitute.org/investigators/springer...
Added: Wed Aug 14 2002, Hits: 326, Reviews: 0 Write review Cached -
Metabolic Labeling of Cell Proteins with [35S]-Methionine
(Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research )
http://medicine.ucsd.edu/hypertension/list_of_prot...
Added: Tue Feb 17 2004, Hits: 1501, Reviews: 0 Write review Cached -
Metabolic Labeling of Cells with 35S
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
http://www.its.caltech.edu/%7Ebjorker/Metabolic_La...
Added: Wed Aug 14 2002, Hits: 1125, Reviews: 0 Write review -
Purification of human mononuclear cells and neutrophils
(Springer Lab, Harvard University)
cell isolation
http://www.cbrinstitute.org/investigators/springer...
Added: Wed Aug 14 2002, Hits: 3560, Reviews: 0 Write review Cached -
Separation of Platelets from Whole Blood
(Springer Lab, Harvard University)
This protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow chamber assays, flow cytometry measurements, etc.
http://www.cbrinstitute.org/investigators/springer...
Added: Wed Aug 14 2002, Hits: 716, Reviews: 0 Write review Cached