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Lamond Lab Protocols: Cells

**Protocols are downloaded as PDF files which can be opened with the free Adobe Acrobat Reader

Establishing stable cell lines

Fusing cells to create heterokaryons

Lysing cells for release of both cytoplasmic and nuclear proteins

Preparation of nuclear extract from plated cells

CFP/YFP FRET measurement

NB: These protocols are intended for use by trained personnel only, in a research laboratory environment.

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Last Updated April 22, 2003