Subcategories
|
Related Categories:
Protocols
- Chemotaxis Assay (Springer Lab, Harvard University)
The purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cell type. Chemotaxis is the ability of a protein to direct the migration of a specific cell. This assay is based on the premise of creating a gradient of the chemotactic agent and allowing cells to migrate through a membrane towards the chemotactic agent. If the agent is not chemotactic for the cell, then the majority of the cells will remain on the membrane. If the agent is chemotactic, then the cells will migrate through the membrane and settle on the bottom of the well of the chemotaxis plate.
http://www.cbrinstitute.org/investigators/springer...
Added: Wed Aug 14 2002, Hits: 2594, Reviews: 1 Read reviews Write review Cached - Microtubule (MT)/Organelle Motility Assays (Salmon Lab, Department of Biology, University of North Carolina at Chapel Hill )
An in vitro assay that uses video-enhanced differential interference contrast (VE-DIC) microscopy to examine the motile interactions between isolated organelle fractions and microtubules
http://www.bio.unc.edu/faculty/salmon/lab/protocol...
Added: Mon Aug 14 2006, Hits: 450, Reviews: 0 Write review Cached -
3D Assay in Matrigel
(Bissell Lab, Berkely Lab)
http://www.lbl.gov/lifesciences/BissellLab/labprot...
Added: Tue Jul 17 2007, Hits: 677, Reviews: 0 Write review -
Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates
(Glycoteck)
his procedure allows detection of specific cell adhesion to glycolipids resolved on TLC plates. The steps include: (1) HPTLC of glycoconjugates, (2) coating the chromatogram with polymer, (3) preblocking the chromatogram to reduce non-specific cell binding, (4) mounting the chromatogram in the acrylic chamber, (5) adding labeled cells, (6) incubating, (7) removal of non-adherent cells by centrifugation, and (8) fixation of adherent cells for detection.
http://www.glycotech.com/protocols/Proto9.html
Added: Tue May 14 2002, Hits: 681, Reviews: 0 Write review Cached -
Cell Adherence Assay
(Laurie Lab, University of Virginia Health Science Center)
Proteins are coated on microtiter plates and cells are added; after the nonbound cells are washed away...
http://www.people.virginia.edu/~gwl6s/protocols/ad...
Added: Tue May 14 2002, Hits: 6659, Reviews: 0 Write review Cached -
Dynamic Flow Assay in a Parallel Plate Flow Chamber
(Glycotek)
Flow assays allow visualization of cell adhesion under well-defined wall shear stress. The visualization of the different events of cell adhesion can be quantified by selective image acquisition and subsequent image processing. Flow assays are uniquely suited to the investigation of adhesive events which occur very rapidly in a time scale shorter than that of most static adhesion assays. In addition, events subsequent to the initial events can be studied such as cell stabilization and spreading giving some insight into the kenetics of particular cell-cell or cell-substrate adhesive behavior.
http://www.glycotech.com/protocols/proto7.html
Added: Tue May 14 2002, Hits: 1338, Reviews: 0 Write review Cached -
Measurement of Cell Adhesion Under Static Conditions
(Glycoteck)
This protocol introduces the use of a liquid-filled wash chamber that separates unbound cells by gravity thereby eliminating uncontrolled shear forces and passage of adherent cells through a liquid/air interface. This protocol is also useful for assaying molecules that promote or inhibit cell adhesion.
http://www.glycotech.com/protocols/Proto8.html
Added: Tue May 14 2002, Hits: 2167, Reviews: 0 Write review Cached -
Motility Assay using VE-DIC Microscopy
(Liz Greene and Steve Henikoff, The Kinesin Home Page)
http://www.proweb.org/kinesin/Methods/VE-DIC.html
Added: Fri Jul 30 2004, Hits: 1135, Reviews: 0 Write review Cached -
Motor Polarity Using Fluorescent Microtubules
(Liz Greene and Steve Henikoff, The Kinesin Home Page)
http://www.proweb.org/kinesin/Methods/Fluorescent_...
Added: Fri Jul 30 2004, Hits: 414, Reviews: 0 Write review Cached -
Preparation of Flexible Polyacrylamide Particles for Phagocytosis
(Yu-li Wang Lab, University of Massachusetts Medical School)
Preparation of protein-coated polyacrylamide beads for phagocytosis assay.
http://ylwang.umassmed.edu/protocol/mc/pabeads.htm
Added: Mon Nov 15 2004, Hits: 637, Reviews: 0 Write review Cached