Top : Cell Biology : Apoptosis

Summary: Cell death can occur by either of two distinct mechanisms, necrosis or programmed cell death (apoptosis). Necrosis is a pathological process which occurs when cells are exposed to a serious physical or chemical insult. Apoptosis is a physiological and controlled process by which unwanted or useless cells are eliminated during development and other normal biological processes. Apoptosis can be detected in populations of cells or in individual cells. Many different methods have been devised to detect apoptosis such as The TUNEL (TdT-mediated dUTP Nick-End Labeling) analysis, ISEL (in situ end labeling), and DNA laddering analysis for the detection of fragmentation of DNA in populations of cells or in individual cells, Annexin-V analysis that measures alterations in plasma membranes, detection of apoptosis related proteins such p53 and Fas.
Related Categories: