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Protocols

• Cajal Body Isolation (Lamond Lab, University of Dundee )
  The Cajal body (CB) is a conserved, dynamic nuclear structure that is implicated in various cellular processes, such as the maturation of splicing small nuclear ribonucleoproteins and the assembly of transcription complexes. Here is the procedure for the large-scale purification of CBs from HeLa cell nuclei, resulting in an ~750-fold enrichment of the CB marker protein p80-coilin. Also available are Quicktime movies demonstrating key steps of the protocol
  http://www.lamondlab.com/f5cbprotocol.htm
  Added: Thu Nov 14 2002, Hits: 79, Reviews: 0  Write review   Cached
• Adrenal Chromaffin Granule (chromaffin vesicle) Preparation  (Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research )
  This method is usually used to prepare granules from freshly obtained (never frozen!) bovine adrenal glands, but can also be used to prepare chromaffin granules from fresh human pheochromocytoma.
  http://hypertension.ucsd.edu/list_of_protocols/17_...
  Added: Tue May 14 2002, Hits: 227, Reviews: 0  Write review   Cached
• Em Observations of Microsomes (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
  http://homepages.gac.edu/~cellab/chpts/chpt7/ex7-2...
  Added: Tue May 14 2002, Hits: 315, Reviews: 0  Write review   Cached
• Lysosome Isolation in Isotonic Sucrose (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
  http://homepages.gac.edu/~cellab/chpts/chpt7/ex7-1...
  Added: Tue May 14 2002, Hits: 440, Reviews: 0  Write review   Cached
• Preparation of cytoplasmic Extracts (The Science Advisory Board)
  Preparation of cytoplasmic extracts for the application in a cell-free system. Cells are grown to 80% confluency, then harvested, washed and disrupted in KPM buffer by freezing-thawing cycles with liquid nitrogen...
  http://www.scienceboard.net/resources/protocols.as...
  Added: Mon Feb 23 2009, Hits: 444, Reviews: 0  Write review   Cached
• Preparation of Rat Liver Cell Cytosol (Salmon Lab, University of North Carolina at Chapel Hill)
  http://www.bio.unc.edu/faculty/salmon/lab/protocol...
  Added: Tue May 14 2002, Hits: 971, Reviews: 0  Write review   Cached
• Preparation of tubulin (Salmon Lab)
  Simple procedure with high yielding
  http://www.bio.unc.edu/faculty/salmon/lab/protocol...
  Added: Tue May 14 2002, Hits: 294, Reviews: 0  Write review   Cached