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121. WGA problems CpG island amplification - (reply: 6)
122. Positive control after 5-Aza treatment? - (reply: 2)
123. methylation analysis - methylation analysis using epitect bisulfite kit from qiagen (reply: 11)
124. How do you determine the linear range of ChIP and input samples? - (reply: 2)
125. need a whole genome methylation protocol - (reply: 2)
126. what to resuspend the DNA pellet after DNA isolation step? - (reply: 4)
127. problem with anti-acetyl H3 antibody - (reply: 11)
128. ChIP sonication problem - (reply: 7)
129. Bisulphite sequencing - Fwd sequence (reply: 1)
130. imprinted genes - which parent? - (reply: 1)
131. WGA problems - Input samples look terrible! - Any advice? (reply: 7)
132. More deep ChIPping woes (Sonication Optimization) - Primary cell line.... (reply: 1)
133. ***MSP Primer Troubleshooting Specificity*** - (reply: 6)
134. Anyone going to Spain in May? - Interplay among genetics, epigenetics and non-coding RNA's (reply: 2)
135. Methylation studies - New to this field... help me (reply: 1)
136. no-antibody control is stronger than my sample! - in ChIP.. (reply: 5)
137. Bisulfite direct sequencing + cloning - (reply: 2)
138. Problem with amplifying bisulfite treated DNA from FFPE tissue - (reply: 3)
139. Phenol/Chlorophorm DNA precipitation after ChIP - (reply: 4)
140. What conc. of ssDNA and BSA? - To reduce background? (reply: 2)
141. Inconsistent bisulfite PCR - (reply: 8)
142. How do you label your amplified DNA? - If you use the Sigma WGA kit... (reply: 6)
143. ESME software - Direct sequencing (reply: 1)
144. ChIP - a couple of Q's about real time validation - Too much background? Compare to IgG or Input? (reply: 1)
145. Serious problem with bisulfite modification - (reply: 3)
146. BSP to test imprinted genes DNA methylation of oocytes - no specific or smear bands! (reply: 4)
147. RT-PCR using formaldehyde cross linked samples - RNA extraction using CHIP crosslinked samples (reply: 1)
148. methylation - primier design (reply: 4)
149. ChIP on peripheral blood samples.... - anyone done it?? (reply: 2)
150. Bisulfite sequencing condition - (reply: 3)
122. Positive control after 5-Aza treatment? - (reply: 2)
123. methylation analysis - methylation analysis using epitect bisulfite kit from qiagen (reply: 11)
124. How do you determine the linear range of ChIP and input samples? - (reply: 2)
125. need a whole genome methylation protocol - (reply: 2)
126. what to resuspend the DNA pellet after DNA isolation step? - (reply: 4)
127. problem with anti-acetyl H3 antibody - (reply: 11)
128. ChIP sonication problem - (reply: 7)
129. Bisulphite sequencing - Fwd sequence (reply: 1)
130. imprinted genes - which parent? - (reply: 1)
131. WGA problems - Input samples look terrible! - Any advice? (reply: 7)
132. More deep ChIPping woes (Sonication Optimization) - Primary cell line.... (reply: 1)
133. ***MSP Primer Troubleshooting Specificity*** - (reply: 6)
134. Anyone going to Spain in May? - Interplay among genetics, epigenetics and non-coding RNA's (reply: 2)
135. Methylation studies - New to this field... help me (reply: 1)
136. no-antibody control is stronger than my sample! - in ChIP.. (reply: 5)
137. Bisulfite direct sequencing + cloning - (reply: 2)
138. Problem with amplifying bisulfite treated DNA from FFPE tissue - (reply: 3)
139. Phenol/Chlorophorm DNA precipitation after ChIP - (reply: 4)
140. What conc. of ssDNA and BSA? - To reduce background? (reply: 2)
141. Inconsistent bisulfite PCR - (reply: 8)
142. How do you label your amplified DNA? - If you use the Sigma WGA kit... (reply: 6)
143. ESME software - Direct sequencing (reply: 1)
144. ChIP - a couple of Q's about real time validation - Too much background? Compare to IgG or Input? (reply: 1)
145. Serious problem with bisulfite modification - (reply: 3)
146. BSP to test imprinted genes DNA methylation of oocytes - no specific or smear bands! (reply: 4)
147. RT-PCR using formaldehyde cross linked samples - RNA extraction using CHIP crosslinked samples (reply: 1)
148. methylation - primier design (reply: 4)
149. ChIP on peripheral blood samples.... - anyone done it?? (reply: 2)
150. Bisulfite sequencing condition - (reply: 3)