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ShRNA Related Discussions
  1. Stability of transient shRNA transfection - (reply: 1)
  2. Problem shrna cloning, bad sequencing results - (reply: 1)
  3. Knockdown with shRNA not stable (Why?) - (reply: 3)
  4. miR30 based shRNA in mouse cells - (reply: 3)
  5. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  6. clonogenic assay for shRNA transfected cells - (reply: 3)
  7. Trouble with shRNA transfection - (reply: 2)
  8. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
  9. To small amount of cells used for shRNA transfection - (reply: 1)
  10. shRNA interference using Lenti virus vector - (reply: 1)
  11. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
  12. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
  13. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
  14. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
  15. Puromycin - shRNA - stable cell line for knock down - (reply: 4)
  16. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
  17. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
  18. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
  19. Converting shRNA sequence to siRNA - (reply: 1)
  20. Oligo primers for shRNA construction - (reply: 1)
  21. Need an effective siRNA/ shRNA sequence for downregulating caveolin-1 - (reply: 2)
  22. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
  23. shRNA to primary keratinocytes - (reply: 1)
  24. si/shRNA sequences against eGFP - (reply: 1)
  25. mir30-based shrna cloning-mismatch - (reply: 1)
  26. BLAST of shRNA sequence - (reply: 2)
  27. protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
  28. shRNA rescue with site directed mutagenesis - oligo design - (reply: 4)
  29. shRNA screening - (reply: 2)
  30. ShRNA transfection - (reply: 1)
  31. shRNA produce virus - (reply: 2)
  32. shRNA depletion and enrichment screening - (reply: 3)
  33. genome wide shrna screen - (reply: 2)
  34. Anyone know Lentivirus ShRNA in vivo knock down - (reply: 3)
  35. Help: protocols for shRNA knockdown effect on Ag-specific Treg differentiation - (reply: 1)
  36. siRNA to shRNA...trouble! - (reply: 1)
  37. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
  38. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  39. how long will it takes to silence target gene after induction of shRNA - (reply: 2)
  40. abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (reply: 2)
  41. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  42. shRNA - (reply: 4)
  43. Problem in shRNA Annelaing ( Help Required ) - (reply: 2)
  44. Problems ligating shRNA primers - Sequencing reveals damaged DNA (reply: 1)
  45. How to do stable knock-down by shRNA advices for beginner - (reply: 1)
  46. non-silencing shRNA control - (reply: 3)
  47. shRNA expression levels - (reply: 4)
  48. Effect of MOI on lentiviral shRNA knockdown - (reply: 1)
  49. knockdown issue...please help... - shRNA pGIPZ vector (reply: 1)
  50. shRNA oligo annealing problem - (reply: 5)
  51. shRNA selection in suspension cell culture - (reply: 6)
  52. shRNA negative control - (reply: 2)
  53. shRNA stable line - need better reduction of target (reply: 8)
  54. retrovirus shRNA - (reply: 1)
  55. ShRNA vectors for other than mammalian systems - shRNA for S. pombe (reply: 6)
  56. shRNA plasmid - which company is good? (reply: 2)
  57. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  58. shRNA stable lines - no stable reduction seen (reply: 3)
  59. In vitro shRNA screening trouble - (reply: 4)
  60. Promoters and vectors for shRNA? The need for specific vectors and promoters? - (reply: 4)
  61. New to shRNA - (reply: 4)
  62. olligo annealing for shRNA cloning - (reply: 20)
  63. inducible shRNA vector system - (reply: 7)
  64. shRNA from SIGMA - (reply: 5)
  65. shRNA transgenic mice - (reply: 1)
  66. Problem in annealing shRNA oligos - (reply: 2)
  67. Which shRNA provider or calculator do YOU use? - (reply: 4)