Summary: Colony PCR is a method used to quickly screen for plasmid inserts directly from E. coli colonies without plasmid preparation. PCR reaction is assembled as standard PCR except the template DNA is bacteria.
( Cindy Troxell)
Colony PCR can be used to identify colonies where your favorite gene (yfg) has been replaced with a marker gene by homologous recombination, and to distinguish homologous recombination events from non-homologous. It can also be used to identify colonies from a tetrad that carry a particular gene replacement. This is not a substitute for Southern blotting, but an adjunct.
Added: Tue May 14 2002, Hits: 4586, Reviews: 0
It works either for E coli and yeast
Added: Tue May 14 2002, Hits: 1774, Reviews: 0
Colony PCR Protocol
(Preuss Lab, University of Chicago)
Added: Thu Feb 12 2009, Hits: 1440, Reviews: 0