Subcategories

Protocols

• Stable Transfection (The Morimoto Lab, Northwestern University)
  Establish stable cell line by lipofectamine transfection.
  http://www.biochem.northwestern.edu/ibis/morimoto/...
  Added: Mon Mar 29 2004, Hits: 28815, Reviews: 0  Write review  
• Calcium Chloride Transfection (The Morimoto Lab, Northwestern University)
  Establish stable cell line by calcium chloride transfection.
  http://www.biochem.northwestern.edu/ibis/morimoto/...
  Added: Mon Mar 29 2004, Hits: 7558, Reviews: 0  Write review  
• Establishment of Stable Transfectant of CHO Lec Cells (Springer Lab, Harvard Medical School)
  Electroporation method
  http://www.cbrinstitute.org/investigators/springer...
  Added: Tue Aug 13 2002, Hits: 4156, Reviews: 0  Write review   Cached
• Generating Stable Cell Lines (Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
  http://www.pharma.ethz.ch/institute_groups/biomacr...
  Added: Mon Nov 11 2002, Hits: 7368, Reviews: 0  Write review   Cached

Trouble Shootings and FAQs

• How to decide antibiotic doses?
• How to determinine a dose response curve for the selectable marker
• Stable transfectants don't give off any fluorescence
• G418 storage and contamination
• Selection of clones during transfection - what is the concentration of G418 to be added to the cell.
• How to establish stable cell lines
• Stable cell lines not overexpressing my gene of interest!
• Time needed to determine the concentration of G418?
• Stable transfection of mouse embryonic stem cells