Protocols
- Picking Cloned Cells
(Sugden Laboratory, McArdle Laboratory for Cancer Research)
A method of using whatman paper to pick clones.
http://mcardle.oncology.wisc.edu/sugden/protocols/...
Added: Wed Nov 23 2005, Hits: 1253, Reviews: 0 Write review Cached -
Cloning Cells by Limiting Dilution
(The Science Advisory Board)
This protocol describes a method that can be used to clone individual cells. This is done by limiting dilution and examination of plates for single cells. Growth factors or selection components may be added to specifically amplify a particulat phenotype required.
http://www.scienceboard.net/resources/protocols.as...
Added: Sun Jun 05 2005, Hits: 2076, Reviews: 0 Write review Cached -
Selection of Transfected Suspension Cells
(Hannun/Obeid Lab, Duke Univ.)
Select stable transfected cells in suspension culture
http://www.duke.edu/web/ceramide/protocols/0008.ht...
Added: Sun Apr 24 2005, Hits: 1646, Reviews: 0 Write review Cached -
Stable Transfection and Cloning Harvesting
(Cell Biology Lab, School of Anatomy, UNSW, Sydney, Australia)
It describes in great detail how to pick up clones using a cloning cylinder, along with general transfection procedures.
http://anatomy.med.unsw.edu.au/cblonly/methods/mh1...
Added: Thu Apr 08 2004, Hits: 5853, Reviews: 0 Write review Cached -
Use of Cloning Cylinders for Harvesting Cell Colonies
(Corning Life Science)
3-page protocol that describes how to isolate and develop a population of cells (a clone) that are the descendants of a single cell.
http://www.corning.com/Lifesciences/technical_info...
Added: Thu Jul 15 2004, Hits: 2597, Reviews: 0 Write review