Stable Transfection/Clone Harvesting Protocols

Cell Cloning by Serial Dilution 96 Well Plates (Corning)
This 3-page protocol describes a simple approach using a 96 well plate to isolate colonies derived from single cells.
http://catalog2.corning.com/Lifesciences/media/pdf...
Added: Wed Feb 11 2009, Hits: 2208, Reviews: 0 Write review
Single Cell Isolation Using Glass Chips (Corning)
This 3-page protocol describes a simple approach using glass coverslip chips to isolate colonies derived from single cells.
http://catalog2.corning.com/Lifesciences/media/pdf...
Added: Wed Feb 11 2009, Hits: 903, Reviews: 0 Write review
Stable Transfection and Cloning Harvesting (Cell Biology Lab, School of Anatomy, UNSW, Sydney, Australia)
It describes in great detail how to pick up clones using a cloning cylinder, along with general transfection procedures.
http://anatomy.med.unsw.edu.au/cblonly/methods/mh1...
Added: Thu Apr 08 2004, Hits: 2737, Reviews: 0 Write review Cached
Use of Cloning Cylinders for Harvesting Cell Colonies (Corning)
3-page protocol that describes how to isolate and develop a population of cells (a clone) that are the descendants of a single cell.
http://catalog2.corning.com/Lifesciences/media/pdf...
Added: Wed Feb 11 2009, Hits: 1314, Reviews: 0 Write review