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Recipe for 10 lt of J/J medium:
To 9 lt of mili-Q water, addAdjust pH to 7.15 and filter sterilize.
- MEM w/o Gln (GIBCO, #11700-077). Pwdr for 10 lt.
- NaHCO3, 27.75g
- glucose: 30 g
- nucleosides: adenosine (Sigma A-4036), 70mg; guanosine (Sigma G-6264), 70mg; cytidine (Sigma C-4654), 70mg; uridine (Sigma U-3003), 70mg; thymidine (Sigma T-1895),24mg
- glutamic acid (G-1626) and asparagine (A-0884); 600 mg each
- sodium pyruvate solution (100X, 100mM, GIBCO #11360-021), 100ml
- nonessential amino acid solution (100X, 10mM, GIBCO #11140-019), 200ml
- Peniciline/Streptomycin (100x) , 100ml