Subcategories

Related Categories:

• Cell Culture

Protocols

• Calcium Phosphate Transfection (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
  Describes transfection, clone picking and screening
  http://www.its.caltech.edu/%7Ebjorker/Ca-P_Transfe...
  Added: Wed Aug 14 2002, Hits: 13658, Reviews: 0  Write review  
• Calcium Phosphate Transfection of Neurons in Primary Culture (Steven Finkbeiner, Departments of Neurology and Physiology, UCSF)
  http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/pro...
  Added: Tue Oct 01 2002, Hits: 2688, Reviews: 0  Write review   Cached
• CaPO4 Transfection for Chromaffin Cells (Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research )
  http://medicine.ucsd.edu/hypertension/list_of_prot...
  Added: Tue Feb 17 2004, Hits: 1506, Reviews: 0  Write review   Cached
• CaPO4 Transformation of Mammalian Cells (Dr. Bart Frank, Arthritis and Immunology Program, Oklahoma Medical Research Foundation)
  Protocol includes DNA and cell preparation, transfection and receipes
  http://omrf.ouhsc.edu/~frank/CAPO4TRANSFECT.html
  Added: Mon Dec 19 2005, Hits: 2921, Reviews: 0  Write review   Cached
• FAQs for Antibiotics (Invitrogen)
  tips for does and preparation of Geneticin (G418)
  http://invitrogen.custhelp.com/cgi-bin/invitrogen....
  Added: Tue May 14 2002, Hits: 3031, Reviews: 0  Write review   Cached
• Generation of recombinant baculoviruses by co-transfection (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
  For co-transfection prepare at least 10mg of highly purified plasmid DNA. Spodoptera frugiperda cells are sensitive to some contaminants found in crude plasmid preparations, which cannot be removed by phenol/chloroform extraction or ethanol precipitation.
  http://www.its.caltech.edu/%7Ebjorker/Baculovirus-...
  Added: Wed Aug 14 2002, Hits: 2124, Reviews: 0  Write review  
• Seeding Cell Cultures for Transfections (Gumbleton Lab, Cardiff University)
  How to plate cells for transfection.
  http://www.cf.ac.uk/phrmy/PCB/LabProtocolsPDFs/Cel...
  Added: Fri Jul 20 2007, Hits: 1839, Reviews: 0  Write review  
• Selection of Transfected Suspension Cells (Hannun/Obeid Lab)
  http://www.musc.edu/bcmb/ceramide/protocols/0008.h...
  Added: Tue May 14 2002, Hits: 3241, Reviews: 0  Write review   Cached
• Transfection normalizing using dot-blot hybridisation (Methods-Online)
  It is a known, that the usage of plasmid expressing reporter gene driven by strong promoter causes problems when one wants to compare different cell lines. This method allowes you to measure directly the amount of the plasmid which entered the cells and therefore provides a reliable reference.
  http://www.methods.info/Methods/Transfection/Trans...
  Added: Wed Sep 04 2002, Hits: 774, Reviews: 0  Write review   Cached
• Transfection of Mammalian Cell (Calcium Phophate Method) (Botwell Lab)
  http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
  Added: Tue May 14 2002, Hits: 5054, Reviews: 0  Write review   Cached

Trouble Shootings and FAQs

• Will co-transfected vectors enter the same cell?
• Difficulty in transfecting U937 suspension cells
• Tips on transfection of suspension cells
• How to do an in vivo transfection?
• How to detect target gene after the transfection?
• Add or do not add penicillian/ streptomycin in trasnfection?
• Non-viral transfection of mesenchymal stem cells (MSCs)
• How to efficiently transfect macrophage
• Best transfection method/reagent for HeLa cells?

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