Top : Molecular Biology : RNA : RNA Extraction and Isolation
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Protocols
  • Total RNA isolation protocol (Evrogen)
    The procedure is suitable for all types of tissues from wide variety of animal species. The method is based on the well-known protocol of Chomczynski and Sacchi (Chomczynski and Sacchi, 1987), except that all steps are performed at neutral pH instead of acidic as was originally suggested.
    http://www.evrogen.com/technologies/RNA-isolation....
    Added: Fri Feb 06 2009, Hits: 1971, Reviews: 0  Write review   Cached
  • RNA and protein extraction from the same tissue (Ivan J Delgado)
    http://ivaan.com/protocols/139.html
    Added: Sun Mar 01 2009, Hits: 3664, Reviews: 0  Write review   Cached
  • DNA/RNA Extraction from the Same Material (Pat Heslop-Harrison, Molecular Cytogenetics, University of Leicester)
    A protocol for extraction of both DNA and RNA from the same material typically plant leaves.
    http://www.le.ac.uk/biology/phh4/dnarna.htm
    Added: Fri Feb 06 2009, Hits: 1988, Reviews: 0  Write review   Cached
  • How to prepare Molecular Biology grade glycogen (Contributed by Roberto Salvi)
    Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage is that it is very expensive (e.g., About 100 dollars/20-40 mg). Here we present a simple and inexpensive protocol to prepare a large amount of glycogen which is suitable for any kind of Molecular Biology application (we routinely use it for RNA work).
    Added: Tue May 14 2002, Reviews: 0  Write review  
  • Single Step RNA Isolation PDF file (Steven Finkbeiner, UCSF)
    http://www.gladstone.ucsf.edu/gladstone/files/fink...
    Added: Fri Feb 20 2009, Hits: 1330, Reviews: 0  Write review