Protocols
- Isolation of poly-A RNA (Ivan J Delgado)
Protocol for isolating poly A+ RNA on cellulose oligo-dT column
http://ivaan.com/protocols/141.html
Added: Tue Mar 03 2009, Hits: 645, Reviews: 0 Write review Cached - mRNA (Poly A+) Extraction Protocol (Mullins Lab, University of Washington)
using Oligo (dT)-cellulose
http://ubik.microbiol.washington.edu/protocols/arr...
Added: Tue Mar 03 2009, Hits: 663, Reviews: 0 Write review Cached - Purification of Poly(A)+ RNA from Total RNA (Hideaki Shiraishi, Kyoto University)
This method uses using Oligotex-dT30 for poly(A)+ RNA purification.
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
Added: Fri Jan 30 2004, Hits: 3223, Reviews: 0 Write review Cached -
Direct Isolation of Poly A+ RNA
(Faulkner-Jones)
This method can be used to isolate mRNA directly from cultured cells by adding the oligo (dT) cellulose to proteinase K-digested crude cell lysate. Some whole tissues can be lysed directly in the proteinase K solution, whilst others, notably pancreas, need prior extraction of total RNA using guanidinium or lithium-urea. The total RNA can then be PolyA+ selected using oligo(dT) cellulose.
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 1454, Reviews: 0 Write review Cached -
Human Tumor mRNA Isolation
(DeRisi Lab, Dept. of Biochemistry & Biophysics, Univ. of California at San Francisco)
Protocol for extraction of mRNA from human tumors. This protocol is for DNA microarray experiment, but can be used for general purposes.
http://www.microarrays.org/pdfs/HumanTumorRNAIsola...
Added: Tue May 14 2002, Hits: 1442, Reviews: 0 Write review -
Isolation Of Poly A+ RNA From RNA Using Oligo-dT Column
(The Pamela Stanley Lab Wiki)
http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
Added: Tue Mar 03 2009, Hits: 203, Reviews: 0 Write review Cached -
Isolation of poly A+ RNA from Total RNA by Oligo (deoxythymidine ) cellulose Chromatography
(Faulkner-Jones)
Total RNA is first isolated from the tissues or cells and then mRNA is isolated by PolyA+ selection using oligo(dT) cellulose. This is necessary for sll tissue sources rich in RNase (and some cell lines).
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 652, Reviews: 0 Write review Cached -
Membrane Bound Polysome Isolation
(DeRisi Lab, Dept. of Biochemistry & Biophysics, Univ. of California at San Francisco)
Protcol for extraction of mRNA associated with membrane bound polysomes via the gradient method. This protocol is for DNA microarray experiment, but can be used for general purposes.
http://www.microarrays.org/pdfs/PolysomeIsolation....
Added: Tue May 14 2002, Hits: 601, Reviews: 0 Write review -
mRNA Preparation
(Breeden Lab, Fred Hutchinson Cancer Research Center)
Prepare mRNA from total RNA using oligo-dT cellulose
http://labs.fhcrc.org/breeden/Methods/yeast%20meth...
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POLY A+ RNA Preparation
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
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Poly A+ RNA Preparation
(Promega)
http://www.promega.com/tbs/tm021/tm021.html#iv
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Poly A+ RNA Preparation Using Proteinase K and SDS
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 501, Reviews: 0 Write review Cached -
Poly A+ RNA Preparation Using TRIzol Reagent
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 854, Reviews: 0 Write review Cached