- Denaturing and Non-denaturing Agarose Gel Electrophoresis of RNA (Ambion)
The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about RNA yield. A denaturing gel system is suggested because most RNA forms extensive secondary structure via intramolecular base pairing, and this prevents it from migrating strictly according to its size. This protocol provides procedures for performing both non-native and native agarose gel electrophoresis of RNA
Added: Sun Jun 05 2011, Hits: 768, Reviews: 0
Analysis of RNA Quality
(Molecular Techniques and Methods)
RNA quality is checked by electrophoresis in agarose gel, by electrophoresis in polyacrylamide gel, or by in vitro translation.
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Checking the quality of total RNA on an agarose gel
(Center for Medical Genomics at the Indiana University School of Medicine)
Detailed non-denaturing agarose gel method of checking RNA quality.
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Methods to Check RNA Integrity
Describes ways of checking RNA quality such as agarose gel electrophoresis, and other alternatives.
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RNA quality control
Any extracted RNA must be devoid of contaminants such as salt, protein, solvents and genomic DNA. Poor quality RNA will lead to problems when performing the reverse transcription and labelling and might affect data quality. We quality control our extracted RNA using gel electrophoresis and optical density measurements. Gel electrophoresis is used to check for Genomic DNA contamination and RNA decay. Optical density is used to assay the RNA yield and to check for contamination by salt, solvent, protein, etc.
Added: Sun Jun 05 2011, Hits: 465, Reviews: 0